Peroxidase-catalysed rupture of the CF bond as and indicator reaction for enzyme immunoassay
Peroxidase-catalysed rupture of the CF bond as and indicator reaction for enzyme immunoassay
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DOI：
10.1016/s0003-2670(00)86569-1
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发表时间：
1989
影响因子：
6.2
通讯作者：
J. Brochot;I. Siddiqi
中科院分区：
文献类型：
--
作者：
J. Brochot;I. Siddiqi
A fluoride ion-selective electrode is utilized as a sensor for the kinetic determination of peroxidase label in enzyme immunoassays. The method is based on a sandwich enzyme-linked immunosorbent assay (ELISA) technique, the peroxidase-catalysed rupture of the covalent CF bond in 4-fluorophenol and the subsequent release of fluoride ions. The determination of human immunoglobulin G (lgG), human α-fetoprotein (AFP) and human placental lactogen (HPL) was investigated. The potentiometric measurement of the rate of release of fluoride ion within 5 min provided a direct correlation with the concentration of analyte present in the sample. The concentration ranges investigated for the analytes were IgG 30 μg l−1–10mg l−1, AFP 5–500 μg l−1and HPL 60 ng l−1–1 mg l−1. Under the given experimental conditions, the detection limits were IgG 30, AFP 12.8 and HPL 1 μg l−1. Replacing the rate method with the fixed-time mode (15–30 min) did not improve the detection limits. The performance of the present method was found to be comparable to that of the spectrophotometric detection technique.
