Supplemental lipoic acid relieves post-weaning diarrhoea by decreasing intestinal permeability in rats
Supplemental lipoic acid relieves post-weaning diarrhoea by decreasing intestinal permeability in rats
复制标题
补充硫辛酸通过降低大鼠肠道通透性缓解断奶后腹泻
DOI：
10.1111/jpn.12427
复制
发表时间：
2017-02-01
影响因子：
2.7
通讯作者：
Ma, X.
中科院分区：
文献类型：
--
作者：
Fan, P.;Tan, Y.;Ma, X.
硫辛酸（LA）是一种天然存在的物质，广泛分布于动物细胞的细胞膜和胞浆中。其细胞内功能包括淬灭自由基和修复氧化蛋白。本研究的目的是使用大鼠模型评估LA对断奶后腹泻的影响。60只断乳大鼠分别饲喂基础饲料或添加LA的饲料，或添加氧化锌（ZnO）的饲料作为阳性对照。LA组和ZnO组的大鼠表现更好，腹泻发生率降低（p < 0.05）。LA和ZnO处理均增强了肠道的稳态和结构，显著降低了尿乳果糖/甘露醇比率（p < 0.05），并增加了肠粘膜紧密连接蛋白闭合蛋白（OCLN）和闭合小带蛋白-1（ZO-1）的表达（p < 0.05）。LA显著提高肠黏膜抗氧化酶活性，降低氧化型谷胱甘肽和丙二醛水平，显著提高还原型谷胱甘肽活性（p < 0.05）。此外，体外研究表明，在IEC-6肠上皮细胞中补充LA显著增强了OCLN和ZO-1在过氧化氢诱导的氧化应激下的表达。总的来说，这些结果表明，LA缓解断奶后腹泻，降低肠道通透性和提高抗氧化指数。
Lipoic acid (LA) is a naturally existing substance which widely distributed in the cellular membranes and cytosol of animal cells. Its intracellular functions include quenching of free radicals and repairing oxidized proteins. The purpose of this study was to evaluate the effects of LA on post-weaning diarrhoea using a rat model. Sixty weaned rats were fed either a basal diet or a LA-supplemented diet, or a zinc oxide (ZnO)-supplemented diet as a positive control. Rats in the LA and ZnO groups had better performance and reduced incidence of diarrhoea (p < 0.05). Both LA and ZnO treatments enhanced intestinal homeostatic and architecture, significantly decreased urinary lactulose to mannitol ratios (p < 0.05) and increased the expression of the intestinal mucosal tight junction proteins occludin (OCLN) and zonula occludens protein-1 (ZO-1) (p < 0.05). LA significantly increased the activities of antioxidant enzymes, and reduced glutathione while decreasing the levels of oxidative glutathione and malondialdehyde in the intestinal mucosa (p < 0.05). Furthermore, an in vitro study indicated that supplementation with LA in IEC-6 intestinal epithelial cells significantly enhanced the expression of OCLN and ZO-1 under hydrogen peroxide-induced oxidative stress. Collectively, these results suggest that LA relieves post-weaning diarrhoea by reducing intestinal permeability and improving antioxidant indices.