MIDBRAIN DOPAMINERGIC CELL LOSS IN PARKINSONS-DISEASE AND MPTP-INDUCED PARKINSONISM - SPARING OF CALBINDIN-D(28K)-CONTAINING CELLS
MIDBRAIN DOPAMINERGIC CELL LOSS IN PARKINSONS-DISEASE AND MPTP-INDUCED PARKINSONISM - SPARING OF CALBINDIN-D(28K)-CONTAINING CELLS
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DOI：
10.1111/j.1749-6632.1992.tb24523.x
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发表时间：
1992-05-11
影响因子：
5.2
通讯作者：
BROOKS, BA
中科院分区：
文献类型：
--
作者：
GERMAN, DC;MANAYE, KF;BROOKS, BA
计算机成像和免疫组织化学染色技术被用来确定哪些中脑多巴胺能（DA）细胞在帕金森病（PD）中幸免于难，以及在用DA神经毒素1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）治疗的动物中幸免于难，以及幸免于难的细胞是否含有钙结合蛋白-D28 k（CaBP）。PD患者的中脑DA神经元比年龄匹配的正常人少55%以上。细胞丢失发生在黑质和红核后区（分别大于61%; DA核A9和A8）和腹侧被盖区（大于42%; DA核A10）。A9核腹侧部分的细胞损失最大。在MPTP处理的食蟹猴中观察到类似的DA细胞丢失模式。在猴和C57 BL/6小鼠中，含CaBP的细胞特异性地位于由PD和MPTP治疗所保留的细胞区域中。这些数据表明，PD和MPTP都破坏了相同的人口中脑DA神经元核A8，A9和A10，也许CaBP保护DA神经元从PD和MPTP引起的细胞死亡。
Computer imaging and immunohistochemical staining techniques were used to determine which midbrain dopaminergic (DA) cells are spared in Parkinson's disease (PD), and in animals treated with the DA neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP), and whether the spared cells contain the calcium-binding protein, calbindin-D28k (CaBP). The PD patients had more than 55% fewer midbrain DA neurons than age-matched normal subjects. The cell loss occurred within the combined substantia nigra and retrorubral area (greater than 61%; DA nuclei A9 and A8, respectively), and the ventral tegmental area (greater than 42%; DA nucleus A10). The cell loss was greatest within the ventral portion of the nucleus A9. A similar pattern of DA cell loss was observed in MPTP-treated Macaca fascicularis monkeys. The CaBP-containing cells were located specifically in the cell regions spared by PD and by MPTP-treatment in both monkeys and C57BL/6 mice. These data suggest that PD and MPTP both destroy the same population of midbrain DA neurons within nuclei A8, A9, and A10, and that perhaps CaBP protects the DA neurons from cell death caused by both PD and MPTP.