Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorum occurs via the type III secretion system integral to the bacterial flagellum.
Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorum occurs via the type III secretion system integral to the bacterial flagellum.
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DOI：
10.1186/1471-2180-9-181
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发表时间：
2009-08-27
期刊：
影响因子：
4.2
通讯作者：
Chuang DY
中科院分区：
文献类型：
--
作者：
Chan YC;Wu HP;Chuang DY
胡萝卜细小杆菌亚种。胡萝卜菌是一种引起软腐病的植物病原性肠杆菌，软腐病的特征是受影响的植物组织发生广泛的浸渍。该物种还产生两种或两种以上称为细菌素的抗菌物质，这增强了它与相关竞争物种的竞争力。然而，低分子细菌素的分泌机制仍不清楚。通过Tn5插入突变，获得了不分泌低分子细菌素(LMWB)的突变株(flhC：：Tn5)Carocin S1。序列分析表明，该插入打乱了该菌株的开放阅读框架2(ORF2)和ORF3。缺失和修复实验表明，ORF2和ORF3都是LMWB胞外分泌所必需的。ORF2和ORF3序列与胡萝卜杆菌亚种的flhD和flhC基因序列具有较高的同源性。这表明它们可能编码了III型鞭毛分泌系统的关键调控成分。因此，Carocin S1的胞外输出是由胡萝卜素杆菌亚种产生的。胡萝卜菌似乎利用细菌鞭毛所必需的III型分泌系统。
Pectobacterium carotovorum subsp. carotovorum is a phytopathogenic enterobacterium responsible for soft rot, a disease characterized by extensive maceration of the affected plant tissue. This species also produces two or more antibacterial substances called bacteriocins, which enhance its competitiveness against related rival species. However, the secretion mechanism for low-molecular-weight bacteriocin is still unknown. A mutant (flhC::Tn5) that did not secrete the low-molecular-weight bacteriocin (LMWB), Carocin S1, was generated by Tn5 insertional mutagenesis. Sequence analysis indicated that this insertion disrupted open reading frame 2 (ORF2) and ORF3 of this strain. Deletion and rescue experiments indicated that ORF2 and ORF3 were both required for extracellular LMWB secretion. The ORF2 and ORF3 sequences showed high homology with the flhD and flhC gene sequences of Pectobacterium carotovorum subsp. atroseptica, Serratia marcescens, Yersinia enterocolitica, and Escherichia coli, indicating that they likely encoded key regulatory components of the type III flagella secretion system. Thus, the extracellular export of Carocin S1 by Pectobacterium carotovorum subsp. carotovorum appears to utilize the type III secretion system integral to bacterial flagella.
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