Effect of nitinol wire surface properties on albumin adsorption
Effect of nitinol wire surface properties on albumin adsorption
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DOI：
10.1016/j.actbio.2006.07.006
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发表时间：
2007-01-01
影响因子：
9.7
通讯作者：
Carroll, W.
中科院分区：
文献类型：
--
作者：
Clarke, B.;Kingshott, P.;Carroll, W.
超弹性形状记忆合金镍钛诺（类似于50%镍和50%钛）是用于支架应用的重要医疗器械材料。然而，特定表面性质在蛋白质吸附中的作用仍然存在争议。在这项研究中，镍钛合金丝的表面粗糙度，疏水性和元素组成对白蛋白吸附的影响进行了研究。特别是，我们证明了在所谓的表面模式中的基质辅助激光解吸/电离飞行时间质谱技术可以用于直接检测白蛋白的导线表面。此外，通过使用I-125标记的白蛋白测定吸附到金属丝上的白蛋白。在所有导丝样品上均检测到白蛋白。表面粗糙度和疏水性似乎对白蛋白吸附没有影响。然而，有一个明确的相关性之间的表面镍和氧浓度和白蛋白吸附量。在金属丝的表面氧化层中具有较高水平的镍和较少氧的样品显示出增加的白蛋白吸附，这可能导致改善的生物相容性。然而，镍是一种有毒物质，可对人体造成许多不良影响，因此表面镍浓度略微富集且不释放镍的镍钛诺可能具有作为医疗器械材料的潜力。(C)2006 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The superelastic, shape memory alloy nitinol (similar to 50% nickel and similar to 50% titanium) is an important medical device material used for stent applications. However, the role specific surfaces properties have in protein adsorption remain controversial. In this study the effects of nitinol wire surface roughness, hydrophobicity and elemental composition upon albumin adsorption are investigated. In particular, we demonstrate that the technique of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the so-called surface mode can be used for the direct detection of albumin on the wire surfaces. In addition, albumin adsorbing to the wires was determined by using I-125-labelled albumin. Albumin was detected on all wire samples. Surface roughness and hydrophobicity appeared to have no effect on albumin adsorption. There was however a clear correlation between the surface nickel and oxygen concentration and the amount of albumin adsorbed. Samples with higher levels of nickel and less oxygen in the surface oxide layer of the wires showed increased albumin adsorption, which could lead to improved biocompatibility. However, nickel is a toxic substance and can cause many adverse effects on humans, and thus nitinol with a slightly enriched surface nickel concentration that does not exhibit nickel release may have potential as a medical device material. (C) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.