Identification and characterization of ERV transcripts in goat embryos
Identification and characterization of ERV transcripts in goat embryos
复制标题
山羊胚胎中 ERV 转录本的鉴定和表征
DOI：
10.1530/rep-18-0336
复制
发表时间：
2019-01-01
期刊：
影响因子：
3.8
通讯作者：
Liu, Jun
中科院分区：
文献类型：
--
作者：
Deng, Ruizhi;Han, Chengquan;Liu, Jun
内源性逆转录病毒（ERV）在哺乳动物基因组中大量存在，可调控邻近基因的表达，在小鼠和人类早期胚胎发育过程中其表达具有动态性和阶段特异性。然而，ERV元件在调控胚胎发育中的功能和机制尚不清楚。在这里，我们利用几种方法来确定ERVs的贡献，化妆和调节转录胚胎基因组激活（EGA）。本研究构建了山羊ERV文库和胚胎RNA-seq文库（IVF_2c和IVF_8c），为进一步研究奠定了基础。对ERV基因附近的GO和KEGG分析表明，ERV基因的某些元件可能与胚胎发育有关。RNA-seq结果与EGA特征一致。为了获得源自ERV序列的转录物，我们用胚胎转录物对ERV序列进行了爆破，并鉴定了在IVF-8 c中而不是在IVF-2c中高度表达的三种IncRNA和一种mRNA（q值
Endogenous retroviruses (ERVs), which are abundant in mammalian genomes, can modulate the expression of nearby genes, and their expression is dynamic and stage-specific during early embryonic development in mice and humans. However, the functions and mechanisms of ERV elements in regulating embryonic development remain unclear. Here, we utilized several methods to determine the contribution of ERVs to the makeup and regulation of transcripts during embryonic genome activation (EGA). We constructed an ERV library and embryo RNA-seq library (IVF_2c and IVF_8c) of goat to serve as our research basis. The GO and KEGG analysis of nearby ERV genes revealed that some ERV elements may be associated with embryonic development. RNA-seq results were consistent with the features of EGA. To obtain the transcripts derived from the ERV sequences, we blasted the ERV sequences with embryonic transcripts and identified three IncRNAs and one mRNA that were highly expressed in IVF-8c rather than in IVF-2c (q-value