Transcriptomic profiling of adaptive responses to ocean acidification
Transcriptomic profiling of adaptive responses to ocean acidification
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DOI：
10.1111/mec.14333
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发表时间：
2017-11-01
影响因子：
4.9
通讯作者：
Raftos, David A.
中科院分区：
文献类型：
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作者：
Goncalves, Priscila;Jones, David B.;Raftos, David A.
一些海洋生物种群似乎具有固有的耐受性或适应压力环境条件的能力，包括与气候变化有关的环境条件。来自B2繁育系的悉尼岩蚝在生理水平上表现出对海洋酸化(OA)的适应能力。为了了解这种生理弹性的分子基础，我们分析了B2牡蛎的鳃转录组，这些牡蛎在不久的将来连续两代暴露在预计的海洋pH中。我们的结果表明，B2牡蛎在面对OA时的独特表现是通过参与多种细胞过程的基因的选择性表达来调节的。随后的高通量定量聚合酶链式反应显示，其中一些转录变化是B2牡蛎所独有的，因此可能与它们对OA的弹性有关。由差异丰富的基因介导的细胞内过程主要涉及细胞周期的控制和细胞内平衡的维持。这些变化可能使B2牡蛎能够防止氧化损伤引起的细胞凋亡，或者通过调节细胞周期来缓解细胞凋亡的影响。对不同世代条件作用的比较分析支持B2和野生型牡蛎具有不同的改变基因表达和对条件作用的反应轨迹的观点。我们的发现揭示了一组广泛的分子过程，这些过程潜在的跨代条件作用和海洋钙化物对OA的潜在弹性。识别压力恢复的机制可以揭示这些生物在快速变化的海洋中生存和茁壮成长的细胞内基础。
Some populations of marine organisms appear to have inherent tolerance or the capacity for acclimation to stressful environmental conditions, including those associated with climate change. Sydney rock oysters from the B2 breeding line exhibit resilience to ocean acidification (OA) at the physiological level. To understand the molecular basis of this physiological resilience, we analysed the gill transcriptome of B2 oysters that had been exposed to near-future projected ocean pH over two consecutive generations. Our results suggest that the distinctive performance of B2 oysters in the face of OA is mediated by the selective expression of genes involved in multiple cellular processes. Subsequent high-throughput qPCR revealed that some of these transcriptional changes are exclusive to B2 oysters and so may be associated with their resilience to OA. The intracellular processes mediated by the differentially abundant genes primarily involve control of the cell cycle and maintenance of cellular homeostasis. These changes may enable B2 oysters to prevent apoptosis resulting from oxidative damage or to alleviate the effects of apoptosis through regulation of the cell cycle. Comparative analysis of the OA conditioning effects across sequential generations supported the contention that B2 and wild-type oysters have different trajectories of changing gene expression and responding to OA. Our findings reveal the broad set of molecular processes underlying transgenerational conditioning and potential resilience to OA in a marine calcifier. Identifying the mechanisms of stress resilience can uncover the intracellular basis for these organisms to survive and thrive in a rapidly changing ocean.