The barley powdery mildew protein kinase C gene, pkc1 and pkc-like gene, are differentially expressed during morphogenesis.
The barley powdery mildew protein kinase C gene, pkc1 and pkc-like gene, are differentially expressed during morphogenesis.
复制标题
大麦白粉病蛋白激酶C基因、pkc1和pkc样基因在形态发生过程中存在差异表达。
作者：
Ziguo Zhang;Gemma D. Priddey;S. Gurr
摘要 蛋白激酶 C 激动剂测定表明，佛波醇酯（佛波醇 12-肉豆蔻酸酯 13-乙酸酯）可引发小麦白粉病 (Blumeria graminis) 的幼苗形态发生并增强 PKC 活性。没有发现霉菌 PKC 活性的拮抗剂，但这些数据推动了对白粉病 pkc 基因的寻找。基于真核蛋白激酶蛋白催化核心内保守的 ATP 结合和激酶结构域设计的寡核苷酸被用作引物，以扩增来自大麦白粉病真菌 graminis 的染色体和 cDNA 片段。分离了三个激酶基因片段（pkc1、pkc-like 和 cpka），并通过“逐步下降”PCR 确定了霉菌 pkc 和 pkc-like 基因的全长基因组序列。 RT-PCR 转录谱显示这三个基因在胚芽形态发生过程中受到差异性调节。
summary Protein kinase C agonist assays revealed the phorbol ester, phorbol 12-myristate 13-acetate, invoked germling morphogenesis and enhanced PKC activity in Blumeria graminis. No antagonist of mildew PKC activity was found but the data fuelled a hunt for powdery mildew pkc genes. Oligonucleotides, designed on the basis of conserved ATP-binding and kinase domains within the catalytic core of eukaryotic protein kinase proteins, were used as primers to amplify chromosomal and cDNA fragments from the barley powdery mildew fungus graminis. Three kinase gene fragments were isolated (pkc1, pkc-like and cpka) and the full length genomic sequences of the mildew pkc and pkc-like genes were determined by 'step down' PCR. RT-PCR transcript profiles showed the three genes to be differentially regulated during germling morphogenesis.
蛋白激酶 Cα 包含两个激活剂结合位点，以相反的亲和力结合佛波酯和二酰甘油。
DOI：
10.1074/jbc.271.9.4627
发表时间：
1996
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Slater,SJ;Ho,C;Kelly,MB;Larkin,JD;Taddeo,FJ;Yeager,MD;Stubbs,CD
通讯作者：
Stubbs,CD