Arabinogalactan proteins 6 and 11 are required for stamen and pollen function in Arabidopsis
Arabinogalactan proteins 6 and 11 are required for stamen and pollen function in Arabidopsis
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DOI：
10.1111/j.1365-313x.2008.03607.x
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发表时间：
2008-11-01
期刊：
影响因子：
7.2
通讯作者：
Zik, Moriyah
中科院分区：
文献类型：
--
作者：
Levitin, Bella;Richter, Dganit;Zik, Moriyah
植物中成功的雄性生殖功能依赖于雄蕊和花粉的正确发育和功能。AGP6和AGP11是两个编码细胞壁相关阿拉伯半乳糖蛋白(AGPs)的同源拟南芥基因。这两个基因都在雄蕊、花粉粒和花粉管中特异表达，表明这些基因可能在男性器官的发育和功能中发挥作用。获得了AGP6和AGP11表达降低的RNAi株。这些，再加上AGP6编码区有点突变的品系，被用来表明AGP6和AGP11功能的丧失导致了育性下降，至少部分是由于抑制了花粉管的生长。我们的结果还表明，AGP6和AGP11在成熟花药释放花粉的过程中起着额外的作用。因此，我们的研究证明了特定的AGPs参与了花粉管的生长和雄蕊的功能。
Successful male reproductive function in plants is dependent on the correct development and functioning of stamens and pollen. AGP6 and AGP11 are two homologous Arabidopsis genes encoding cell wall-associated arabinogalactan glycoproteins (AGPs). Both genes were found to be specifically expressed in stamens, pollen grains and pollen tubes, suggesting that these genes may play a role in male organ development and function. RNAi lines with reduced AGP6 and AGP11 expression were generated. These, together with lines harboring point mutations in the coding region of AGP6, were used to show that loss of function in AGP6 and AGP11 led to reduced fertility, at least partly as a result of inhibition of pollen tube growth. Our results also suggest that AGP6 and AGP11 play an additional role in the release of pollen grains from the mature anther. Thus, our study demonstrates the involvement of specific AGPs in pollen tube growth and stamen function.