The properties of hub proteins in a yeast‐aggregated cell cycle network and its phase sub‐networks
The properties of hub proteins in a yeast‐aggregated cell cycle network and its phase sub‐networks
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DOI：
10.1002/pmic.200900053
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发表时间：
2009-10
期刊：
影响因子：
3.4
通讯作者：
Xiaomei Wu;Jie Guo;Da‐Yong Zhang;Kui Lin
中科院分区：
文献类型：
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作者：
Xiaomei Wu;Jie Guo;Da‐Yong Zhang;Kui Lin
目前，大多数关于相互作用蛋白尤其是中枢特性的研究主要基于二元相互作用的聚合网络。在这里，通过将酵母聚集的细胞周期网络投影为四个阶段（G1、S、G2 和 M）子网络，我们探索了聚集网络中枢的特性。我们发现，正如预期的那样，只有 69%（149 个中的 103 个）的集线器仍然在四个子网络中的至少一个中充当集线器。有趣的是，那些在任何阶段都不是枢纽的人（19%）往往执行多种功能，并且其网络地位与其他人不同。我们还分析了动态和静态枢纽之间的潜在生物学特征。最后，我们研究了两种复合物的动态特性，即后期促进复合物和染色质重塑复合物。后期促进复合物的动态功能通过其在有丝分裂细胞周期的不同阶段与CDC28的相互作用来体现。当其动态成分ARP7在S期周期性表达并在染色质重塑中发挥作用时，染色质重塑复合物就被组装起来，这可以反映蛋白质复合物的即时组装。总之，分解聚合网络和表征分解子网络中的枢纽的研究有助于进一步理解酵母细胞周期的动态功能。
Currently, most researches on the properties of interacting proteins, hubs in particular, are mainly based on an aggregated network of binary interactions. Here, by projecting a yeast‐aggregated cell cycle network into four phase (G1, S, G2 and M) sub‐networks, we explored the properties of the hubs of the aggregated network. We found, as expected that only 69% (103 of 149) of hubs still act as hubs in at least one of the four sub‐networks. Interestingly, those (19%) that are not hubs in any phase tend to perform multiple functions and differ from the others in their network positions. We also analyzed the potential biological features among the dynamic and static hubs. Finally, we investigated the dynamic properties of two complexes, the anaphase‐promoting complex and the chromatin‐remodeling complex. The dynamic function of the anaphase‐promoting complex is represented by its interactions with CDC28 at different stages of mitotic cell cycle. The chromatin‐remodeling complex is assembled when its dynamic component, ARP7, is periodically expressed in S phase to function in chromatin remodeling, which could reflect the just‐in‐time assembly of protein complexes. In conclusion, the study of decomposing the aggregated network and characterizing the hubs in decomposed sub‐networks is useful for further understanding the dynamic functions of yeast cell cycle.