Design and characterization of optical nanorulers of single nanoparticles using optical microscopy and spectroscopy.
Design and characterization of optical nanorulers of single nanoparticles using optical microscopy and spectroscopy.
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作者：
Nallathamby PD;Huang T;Xu XH
目前传统的成像方法无法在纳米尺度上确定单个纳米粒子在溶液和活体中的大小，这限制了纳米粒子的应用。在这项研究中，我们发展了一种新的成像校准方法来表征溶液中单个纳米银的尺寸，方法是利用暗场光学显微镜和光谱(DFOMS)测量单个纳米银纳米颗粒的尺寸依赖的散射局域表面等离子体共振(LSPR)光谱和散射强度。我们合成了直径在2~110 nm之间的近球形的银纳米粒子，用高分辨电子显微镜表征了单个纳米粒子的尺寸，用DFOMS表征了单个纳米粒子的LSPR光谱和散射强度。我们建立了激光共聚焦共振光谱的峰值波长(λ_(Max))或单个纳米粒子的散射强度随其尺寸变化的校准曲线。这些校准曲线允许我们通过使用DFOMS测量单个NPs的LSPR光谱或散射强度来确定1 nm分辨率的单个NPs的尺寸。这些新方法使我们能够创建单个Ag纳米粒子的光学纳米尺子(校准曲线)，用于使用光学显微镜以纳米分辨率实时成像和测量多个单个纳米粒子在溶液中的尺寸。现在，人们可以使用这些新的成像校准方法来实时研究和表征溶液和活体中的单个NPs，以实现广泛的应用。
Current conventional imaging methods cannot determine sizes of single nanoparticles (NPs) in solution and living organisms at nanometer scale, which limits the applications of NPs. In this study, we developed new imaging calibration approaches to characterize the sizes of single Ag NPs in solution at nanometer resolution by measuring their size-dependent scattering localized-surface-plasmon-resonance (LSPR) spectra and scattering intensity using dark-field optical microscopy and spectroscopy (DFOMS). We synthesized nearly spherical shape Ag NPs, ranging from 2 to 110 nm in diameter, and characterized the sizes of single NPs using high-resolution transmission electron microscopy, and the LSPR spectra and scattering intensity of single NPs using DFOMS. We constructed calibration curves of the peak wavelength (λmax) of LSPR spectra or scattering intensity of single NPs versus their sizes. These calibration curves allow us to determine the sizes of single NPs at 1 nm resolution by measuring the LSPR spectra or scattering intensity of single NPs using DFOMS. These new approaches enable us to create optical nano rulers (calibration curves) of single Ag NPs for simultaneously imaging and measuring sizes of multiple single NPs in solution in real-time at nanometer resolution using optical microscopy. One can now use these new imaging calibration approaches to study and characterize single NPs in solution and living organisms in real time for a wide variety of applications.
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影响因子：
16.6
作者：
Xia, Younan;Xiong, Yujie;Lim, Byungkwon;Skrabalak, Sara E.
通讯作者：
Skrabalak, Sara E.
影响因子：
--
作者：
Pastoriza-Santos, Isabel;Liz-Marzan, Luis M.
通讯作者：
Liz-Marzan, Luis M.
影响因子：
3.3
作者：
Haes, AJ;Zhao, J;Van Duyne, RP
通讯作者：
Van Duyne, RP
影响因子：
17.1
作者：
Lee, Kerry J.;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy
影响因子：
2.9
作者：
Tabor, Christopher;Murali, Raghunath;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.