A non-invasive method based on low-field NMR to analyze the quality changes in caviar from hybrid sturgeon (Huso dauricus, Acipenser schrenckiid)
A non-invasive method based on low-field NMR to analyze the quality changes in caviar from hybrid sturgeon (Huso dauricus, Acipenser schrenckiid)
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基于低场核磁共振的非侵入性方法分析杂交鲟（Huso dauricus、Acipenser schrenckiid）鱼子酱的品质变化
DOI：
10.1111/jfpp.13256
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发表时间：
2017-12-01
影响因子：
2.5
通讯作者：
Tan, Mingqian
中科院分区：
文献类型：
--
作者：
Huang, Linlin;Song, Yukun;Tan, Mingqian
开发了一种非侵入性的LF-NMR方法来监测在-4摄氏度下储存11个月的鱼子酱的质子动力学。T-2弛豫谱在0.54-0.75、20.61-30.78和147.24-307.37 ms处分别出现三个峰，而T-1在167.16-217.31 ms处只出现一个峰。T-21和T-22组分在储存期间保持不变，而T-23则无规律地变化。核磁共振分析表明，在盐处理过程中，水分在渗透压梯度的作用下以自由水的形式通过细胞外空间迁移。鱼子酱中的水分和脂肪含量在整个储存时间内保持不变。在11个月的储存期间，TVB-N值有增加的趋势。通过颜色测量来评价鱼子酱的质量变化。实际应用鱼子酱是一种昂贵的水产品，不仅含有蛋白质，而且含有人体必需的氨基酸、微量元素、维生素和高不饱和脂肪酸。在鱼子酱的加工和贮藏过程中，对鱼子酱品质变化的监测和分析是必不可少的。在此，开发并测试了低场核磁共振（LF-NMR）和磁共振成像（MRI）方法，以评估鱼子酱在-4 ℃冷冻保存长达11个月期间的质子变化。使用低频核磁共振和磁共振成像技术是快速，无创，廉价，可视化的结果是预期的。该方法具有较强的普适性，可用于鱼子酱贮藏过程中质子变化的无创、快速监测。
A noninvasive LF-NMR method was developed to monitor proton dynamics on caviar stored at -4 degrees C for 11 months. T-2 relaxation spectra revealed three peaks at 0.54-0.75, 20.61-30.78, and 147.24-307.37 ms, respectively, whereas T-1 showed only one peak around 167.16-217.31 ms. The T-21 and T-22 components remained unchanged during the storage period, while T-23 was irregularly changed. NMR analysis demonstrated the moisture migrated through the extracellular spaces as free water under the action of osmotic pressure gradient during the salting treatment. Moisture and fat contents in caviar remained unchanged during the overall storage time. There was a trend for increased TVB-N values over the 11 months storage. Color measurement was carried out to evaluate the quality change of the caviar. The NMR parameters clearly showed good correlation with moisture.Practical applicationsCaviar is one of the most expensive fishery products which not only contain protein, but also essential amino acids, trace elements, vitamins and high unsaturated fatty acids. The monitoring and analysis of the quality changes of caviar are essential during the period of caviar processing and storage. Herein, the low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) methods were developed and tested to assess the proton changes of caviar during cryopreservation at -4 degrees C for as long as 11 months. The use of LF-NMR and MRI technique is fast, noninvasive, inexpensive, and visualization results are expected. This approach developed in this study is quite general and can be applied to monitor the proton changes of caviar storage in a noninvasive and fast way.