Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization
Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization
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DOI：
10.2174/138620709789104915
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发表时间：
2009-09-01
影响因子：
1.8
通讯作者：
Tan, Hong
中科院分区：
文献类型：
--
作者：
Concepcion, Joy;Witte, Krista;Tan, Hong
生物分子相互作用的分析是药物开发过程中的关键。无标记的生物传感器方法提供关于结合、动力学、浓度和相互作用的亲和力的信息。这些技术提供了在不使用标记的情况下对固定化配体（例如受体）与溶液中的分析物之间的相互作用的实时监测。生物传感器设计和使用生物层干涉法（BLI）检测的进展提供了一个简单的平台，可以在不使用流动池的情况下对生物分子相互作用进行无标记监测。我们回顾了BLI在各种研究和开发环境中的应用，用于定量抗体和蛋白质以及测量动力学参数。
The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.