Neurons derive from the more apical daughter in asymmetric divisions in the zebrafish neural tube
Neurons derive from the more apical daughter in asymmetric divisions in the zebrafish neural tube
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DOI：
10.1038/nn.2547
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发表时间：
2010-06-01
影响因子：
25
通讯作者：
Clarke, Jonathan D. W.
中科院分区：
文献类型：
--
作者：
Alexandre, Paula;Reugels, Alexander M.;Clarke, Jonathan D. W.
在发育中的中枢神经系统中，细胞的不对称分裂对于维持分化神经元的平衡生产和更新神经前体细胞种群至关重要。在无脊椎动物中，这一过程依赖于祖先分裂期间命运决定因素的不对称遗传。人们普遍认为，类似的机制是脊椎动物不对称命运分裂的基础，但缺乏令人信服的证据证明这一点。我们使用完整的斑马鱼胚胎中枢神经系统中单个祖细胞的活体成像来验证这一假设。我们发现，亚细胞结构域的不对称遗传与不对称子代命运密切相关，我们的结果揭示了这一过程的一个意想不到的特征。注定要成为神经元的子细胞来自两个子代中更顶端的那个，而更基本的子代继承了基底突，并补充了顶端的祖细胞池。
In the developing CNS, asymmetric cell division is critical for maintaining the balanced production of differentiating neurons while renewing the population of neural progenitors. In invertebrates, this process depends on asymmetric inheritance of fate determinants during progenitor divisions. A similar mechanism is widely believed to underlie asymmetrically fated divisions in vertebrates, but compelling evidence for this is missing. We used live imaging of individual progenitors in the intact zebrafish embryo CNS to test this hypothesis. We found that asymmetric inheritance of a subcellular domain is strongly correlated with asymmetric daughter fates and our results reveal an unexpected feature of this process. The daughter cell destined to become a neuron was derived from the more apical of the two daughters, whereas the more basal daughter inherited the basal process and replenished the apical progenitor pool.