Action of FdUrd and dCyd on the incorporation of brdurd in Chinese hamster somatic cell DNA and the isolation of auxotrophic mutants
Action of FdUrd and dCyd on the incorporation of brdurd in Chinese hamster somatic cell DNA and the isolation of auxotrophic mutants
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FdUrd和dCyd对中国仓鼠体细胞DNA中brdurd掺入的作用及营养缺陷型突变体的分离
DOI：
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发表时间：
1977
影响因子：
5.6
通讯作者：
J. Thirion
中科院分区：
文献类型：
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作者：
Romain Labrecque;J. Thirion
在溴脱氧尿苷、脱氧胞苷和氟脱氧尿苷存在下生长的中国仓鼠体细胞比在仅溴脱氧尿苷存在下生长的细胞在其DNA中掺入更多的溴脱氧尿苷。因此，它们变得对光照射更敏感。我们的数据表明，0.05 mM-0.2 mM溴脱氧尿苷，0.05 mM脱氧胞苷和10 μg/ml氟脱氧尿苷是选择中国仓鼠体细胞突变体的最佳组合之一。脯氨酸、肌醇或两者的营养菌因此以高频率分离。
Chinese hamster somatic cells grown in the presence of bromodeoxyuridine, deoxycytidine and fluorodeoxyuridine incorporate more bromodeoxyuridine in their DNA than cells grown in the presence of bromodeoxyuridine alone. Thus they become more sensitive to light irradiation. Our data suggest that 0.05 mM–0.2 mM bromodeoxyuridine, 0.05 mM deoxycytidine and 10 mμg/ml fluorodeoxyuridine is one of the best possible combinations for the selection of Chinese hamster somatic cells mutants. Auxotrophs for proline, inositol or both were thus isolated at high frequency.