Improved Method to Retain Cytosolic Reporter Protein Fluorescence While Staining for Nuclear Proteins
Improved Method to Retain Cytosolic Reporter Protein Fluorescence While Staining for Nuclear Proteins
复制标题
DOI：
10.1002/cyto.a.22451
复制
发表时间：
2014-07-01
期刊：
影响因子：
3.7
通讯作者：
Kurschus, Florian C.
中科院分区：
文献类型：
--
作者：
Heinen, Andre P.;Wanke, Florian;Kurschus, Florian C.
使用当前可用的方法，转录因子 (TF) 的染色以及荧光报告蛋白的保留会因荧光损失而受到阻碍。本研究表明，当前的 TF 染色方案不会破坏荧光蛋白 (FP)，但固定不足以将 FP 保留在透化细胞的细胞质中。在本文中，详细阐述了一个简单而可靠的方案，该方案允许有效的 TF 和细胞因子染色，同时将 FP 保留在固定细胞内。 (C) 2014 年国际细胞计数促进会
Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins is hindered by loss of fluorescence using current available methods. In this study, it is shown that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining while retaining FPs inside fixed cells. (C) 2014 International Society for Advancement of Cytometry