Hydrazine effects on vertebrate cells in vitro.
Hydrazine effects on vertebrate cells in vitro.
复制标题
肼对体外脊椎动物细胞的影响。
DOI：
10.1016/0041-008x(80)90100-3
复制
发表时间：
1980
影响因子：
3.8
通讯作者：
M. Berns
中科院分区：
文献类型：
--
作者：
A. Siemens;M. Kitzes;M. Berns
本研究旨在阐明肼对四种已建立的脊椎动物组织培养细胞系（大鼠袋鼠肾、蟾爪肾、人二倍体成纤维细胞和中国仓鼠细胞）和新生大鼠心肌细胞原代培养的细胞效应。细胞暴露于不同浓度的肼（0.001至10毫米）不同的时间。由此得到的生长和形态数据揭示了一个可能的联氨作用位点。在所有的细胞系中，低浓度的肼（0.01 ~ 0.1 mm）抑制了种群的生长。细胞生长最初受到抑制，但即使在培养基中加入新鲜的联氨，细胞也最终恢复到正常的log相生长。在较高浓度（0.5 ~ 2.0 mm）时，联氨是致死的。大多数细胞类型在0.01 mm肼浓度下首先表现为群体生长抑制，但致死浓度随细胞类型不同而不同。用联氨处理的培养物产生了大量的巨大的多核细胞。使用[3H]胸苷的放射自显影研究证实，大的多核细胞是细胞融合的结果。在肼处理的细胞培养中，细胞融合的增加暗示细胞表面可能是一个靶点。扫描电子显微镜证实了对照和肼处理细胞之间的浓度相关表面差异。进一步的膜研究考察了肼对培养心肌细胞收缩和细胞间自发电活动的影响，表明肼也以浓度和时间依赖性的方式改变了这些膜相关活动。
This study was designed to elucidate the cellular effects of hydrazine on four established tissue culture vertebrate cell lines (rat kangaroo kidney, Xenopus toad kidney, human diploid fibroblast, and Chinese hamster cells) and primary cultures of neonatal rat myocardial cells. Cells were exposed to hydrazine in various concentrations (0.001 to 10 mm) for varying time periods. The resulting growth and morphological data revealed a possible site of hydrazine action. In all cell lines tested, population growth was depressed by low concentrations of hydrazine (0.01 to 0.1 mm). Cell growth was initially depressed, but it eventually returned to normal log phase growth even when fresh hydrazine was added to the culture medium. At higher concentrations (0.5 to 2.0 mm), hydrazine was lethal. Most cell types first showed population growth depression at 0.01 mm hydrazine, but the lethal concentration varied with the cell type. Cultures treated with hydrazine yielded a significantly higher number of giant, multinucleated cells. Autoradiography studies employing [3H]thymidine confirmed that the large, multinucleated cells resulted from cell fusion. The increase in cell fusion in hydrazine treated cell cultures implicated the cell surface as a possible target site. Scanning electron microscopy confirmed concentration related surface differences between control and hydrazine-treated cells. Further membrane studies examining the effects of hydrazine on the contractile and intercellular spontaneous electrical activity of myocardial cells in culture indicated that hydrazine also altered these membrane-related activities in a concentration and time-dependent manner.