Differential growth control of normal, preneoplastic and neoplastic Syrian hamster embryo cells in serum-free media.
Differential growth control of normal, preneoplastic and neoplastic Syrian hamster embryo cells in serum-free media.
复制标题
无血清培养基中正常、肿瘤前和肿瘤叙利亚仓鼠胚胎细胞的差异生长控制。
DOI：
10.1093/carcin/14.4.579
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发表时间：
1993
期刊：
影响因子：
4.7
通讯作者：
Iype,PT
中科院分区：
文献类型：
--
作者：
Kaighn,ME;Kumari,HL;Spalding,JW;Barrett,JC;Iype,PT
这项工作的目的是开发一种无血清培养基，支持继代叙利亚仓鼠胚胎（SHE）细胞的生长，适合用于形态转化试验（MTA）。目前的测定采用高水平的胎牛血清，每一批必须选择其在测定中的功效。为了避免与使用血清相关的问题，开发了两种无血清培养基（CGM-4和CGM-9）。使用的方法是用更明确的激素和生长因子代替血清。选择低NaHCO3（pH 6.7）的DME/F12作为基础营养培养基，因为有报道称这种pH可以促进转化。两种无血清培养基均支持正常继发性SHE细胞的克隆高密度生长。这些培养基也被发现支持几种已建立的SHE细胞系的生长，表现出肿瘤进展的连续阶段。然而，观察到单个细胞系对CGM-4和CGM-9的显著差异反应，而在血清补充培养基中，所有三种细胞系的反应相似。这些结果提出了希望，CGM-4和CGM-9将有助于研究在转化和进展过程中改变的自分泌功能，以及用于转化试验本身。
The objective of this work was to develop a serum-free medium that supported the growth of secondary Syrian hamster embryo (SHE) cells suitable for use in the morphological transformation assay (MTA). The current assay employs high levels of fetal bovine serum, each lot of which must be selected for its efficacy in the assay. To circumvent the problems associated with the use of serum, two serum-free media (CGM–4 and CGM–9) were developed. The approach used was to replace serum with more defined hormones and growth factors. DME/F12 with low NaHCO3(pH 6.7) was chosen as a basal nutrient medium since this pH has been reported to give enhanced transformation. Both serum-free media supported the clonal high density growth of normal secondary SHE cells. These media were also found to support the growth of several established SHE cell lines exhibiting successive stages of neoplastic progression. However, significant differential responses of the individual cell lines to CGM-4 and CGM-9 were observed, whereas, in serum-supplemented medium all three lines responded similarly. These results raise the hope that CGM-4 and CGM-9 will be useful for studies of altered autocrine function during transformation and progression as well as for use in the transformation assay itself.