Assessing the role of mitonuclear interactions on mitochondrial function and organismal fitness in natural Drosophila populations
Assessing the role of mitonuclear interactions on mitochondrial function and organismal fitness in natural Drosophila populations
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DOI：
10.1101/2023.09.25.559268
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
S. Bettinazzi;J. Liang;E. Rodriguez;M. Bonneau;R. Holt;B. Whitehead;D. Dowling;N. Lane;M. Camus
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文献类型：
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作者：
S. Bettinazzi;J. Liang;E. Rodriguez;M. Bonneau;R. Holt;B. Whitehead;D. Dowling;N. Lane;M. Camus
线粒体代谢受一系列酶复合物的调节，其功能取决于由线粒体和核基因组编码的蛋白质和RNA之间的有效相互作用。这些上位相互作用反过来又对环境高度敏感。许多研究发现，线粒体单倍型频率的各种类群与纬度或海拔高度，导致假设线粒体基因组可能响应于热选择，并有助于当地的适应。我们使用了由来自澳大利亚东海岸渐变群极端的本地（共适应）种群组成的黑腹果蝇小组，并生成了有丝分裂核胞质杂种种群。我们的研究结果表明，线粒体的相互作用在胞质杂种线，涉及有氧能力和关键健身方面，如繁殖，生长和生存之间的权衡明显的表型影响。总的来说，我们的研究表明，自然发生的线粒体破坏可以对表型产生有意义的影响，可能影响未来的生态适应和种群持久性。
Mitochondrial metabolism is regulated by a series of enzyme complexes, whose function depends on effective interactions between proteins and RNA encoded by the mitochondrial and nuclear genomes. These epistatic interactions are in turn highly sensitive to the environment. Many studies have found that mitochondrial haplotype frequencies of various taxa associate with latitude or altitude, leading to the hypothesis that mitochondrial genomes may respond to thermal selection and contribute to local adaptation. We used a Drosophila melanogaster panel comprising native (coadapted) populations from the extremes of the Australian east-coast cline, and generated mitonuclear cybrid populations. Our results indicate a strong phenotypic impact of mitonuclear interactions in cybrid lines, involving an apparent trade-off between aerobic capacity and key fitness aspects such as reproduction, growth, and survival. Overall, our study shows that naturally-occurring mitonuclear disruptions can have a meaningful impact on phenotypes, potentially influencing future ecological adaptation and population persistence.