Evaluation of autoantibodies against vimentin and α-enolase in rheumatoid arthritis patients.
Evaluation of autoantibodies against vimentin and α-enolase in rheumatoid arthritis patients.
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DOI：
10.5114/reum.2020.101276
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Saghiri R
中科院分区：
文献类型：
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作者：
Ebrahimi-Rad M;Khatami S;Akhbari H;Mahmoudzadeh-Niknam H;Valadbeigi S;Mahmoudi M;Jamshidi A;Riazi-Rad F;Saghiri R
类风湿性关节炎（RA）被归类为一种自身免疫性疾病，全球发病率为0.2-1%。据报道，在RA人群中产生各种自身抗体，特别是针对瓜氨酸肽。在各种RA候选诊断标志物中，瓜氨酸化蛋白对RA的诊断和预后都具有最高的特异性和敏感性。抗突变瓜氨酸蛋白和α-烯醇化酶是一类新的RA早期检测自身抗体。从RA患者中采集的45份血清样本和19份滑液样本被认为是美国风湿病学会的标准，健康受试者提供的20份血清样本和10份滑液样本作为对照组。采用酶联免疫吸附试验（ELISA）检测RA患者血清和SF中抗瓜氨酸蛋白抗体（ACPA）、抗突变瓜氨酸蛋白肽（MCV）和抗α-烯醇化酶的含量。为了评估疾病活动性和关节破坏，我们使用基于红细胞沉降率（ESR）的28个关节的疾病活动性评分（DAS28）。此外，为了测定波形蛋白和α-烯醇化酶的分子量，采用10% SDS-PAGE电泳，如上所述。RA患者血清抗α-烯醇化酶水平显著高于健康人群（4.49±0.20 ng/ml vs. 0.76±0.12 ng/ml） （p < 0.001）。α-烯醇化酶水平与类风湿因子（RF）和c反应蛋白（CRP）水平有直接关系。ACPA阳性和阴性患者的ESR平均值分别为38.2±22.6 mm/h和9.2±5.8 mm/h （p < 0.0001）。平均DAS28-ESR为3.3。RA患者血清抗mcv水平（244.6±53.3 U/ml）高于健康组（148.73±71.8）（p < 0.0001）。患者SF中抗mcv水平为687.5±148.4 U/ml。综上所述，抗MCV自身抗体和α-烯醇化酶是RA患者血清和SF升高的两个重要标志物，对RA疾病的诊断具有特异性。
Rheumatoid arthritis (RA) is categorized as an autoimmune disease with a frequency of 0.2–1% worldwide. It is reported that various autoantibodies are produced in the RA population, particularly against citrullinated peptides. Among various candidate markers for RA diagnosis, the citrullinated proteins have the highest specificity and sensitivity for both diagnosis and prognosis of RA. Anti-mutated citrullinated vimentin and α-enolase constitute a new class of autoantibodies for early detection of RA. 45 serum samples and 19 synovial fluid (SF) specimens collected from RA patients were considered for American College of Rheumatology criteria and 20 serum samples and 10 SF specimens were provided from healthy subjects as a control group. To assess the quantity of anti-citrullinated protein antibodies (ACPA), anti-mutated citrullinated vimentin (MCV) and anti-α-enolase in the serum and SF of RA patients were determined by the enzyme-linked immunosorbent assay (ELISA) method. For the evaluation of disease activity and joint destruction, we used the Disease Activity Score of 28 joints based on erythrocyte sedimentation rate (ESR) Disease Activity Score 28 (DAS28). Furthermore, to measure the molecular weight of vimentin and α-enolase, electrophoresis on 10% SDS-PAGE was performed as described before. The anti-α-enolase level among serum samples from RA patients was significantly higher than in healthy subjects (4.49 ±0.20 ng/ml vs. 0.76 ±0.12 ng/ml) (p < 0.001). There was a direct relation between α-enolase quantity and (rheumatoid factor) RF and C-reactive protein (CRP) levels. The mean ESR value in positive and negative ACPA patients was 38.2 ±22.6 mm/h and 9.2 ±5.8 mm/h respectively (p < 0.0001). The mean DAS28-ESR was 3.3. The level of anti-MCV in the serum of RA patients (244.6 ±53.3 U/ml) was higher than in serum of the healthy group (148.73 ±71.8) (p < 0.0001). The level of anti-MCV in the SF of patients was 687.5 ±148.4 U/ml. In conclusion, both autoantibodies against MCV and α-enolase are two important markers that increase in serum and SF of RA patients and are specific for diagnosis of RA disease.