The Sugar Transporter MST1 Is Involved in Colonization of Rhizosphere and Rhizoplane by Metarhizium robertsii.
The Sugar Transporter MST1 Is Involved in Colonization of Rhizosphere and Rhizoplane by Metarhizium robertsii.
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糖转运蛋白 MST1 参与罗氏绿僵菌在根际和根面的定植
DOI：
10.1128/msystems.01277-21
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发表时间：
2021-12-21
期刊：
影响因子：
6.4
通讯作者：
Fang W
中科院分区：
文献类型：
--
作者：
Dai J;Mi W;Wu C;Song H;Bao Y;Zhang M;Zhang S;Fang W
超过90%的维管植物物种与真菌有着密切的共生关系，这对陆地生态系统产生了巨大的影响。植物共生真菌是由光合产物支持的，但真菌利用植物源碳源的机制尚不清楚。摘要人们普遍认为，植物共生真菌受到光合产物的支持;然而，人们对根际真菌利用植物源糖的分子机制知之甚少。在昆虫致病性和植物共生真菌罗伯茨绿僵菌，我们以前表明，利用低聚糖的转运蛋白MRT（绿僵菌棉子糖转运蛋白）是重要的根际竞争力。在这项研究中，我们确定了一种新的单糖转运蛋白（MST 1），它参与根际的定殖，并与MRT一起定殖根际。MST 1不参与M.罗伯茨。MST 1是一种H+同向转运体，能够转运广谱单糖，包括葡萄糖、山梨糖、甘露糖、鼠李糖和果糖。Mst 1基因的缺失损害了在含有它可以运输的糖的培养基中的萌发和菌丝生长。MST 1的同源物广泛存在于许多真菌中，包括植物共生体如木霉属（Trichoderma spp.）以及菌根真菌和植物病原体如镰刀菌属（Fusarium spp.）这项工作大大推进了对植物和真菌之间共生关系发展的认识。90%以上的维管植物物种与真菌有着密切的共生关系，这对陆地生态系统产生了巨大的影响。植物共生真菌是由光合产物支持的，但真菌利用植物源碳源的机制尚不清楚。在真菌罗伯茨绿僵菌中，我们发现了一种新的单糖转运蛋白（MST 1），它是一种H+同向转运蛋白，可以转运广谱单糖，包括葡萄糖、山梨糖、甘露糖、鼠李糖和果糖。MST 1参与根面的定殖，并与先前表征的寡糖转运蛋白MRT相加作用以定殖根际。在许多真菌中发现了MST 1的同源物，包括植物共生体和植物病原体，这表明MST 1同源物对植物来源的糖的利用对于其他真菌与各自的植物宿主发展共生或寄生关系也很重要。
Over 90% of all vascular plant species develop an intimate symbiosis with fungi, which has an enormous impact on terrestrial ecosystems. It is widely recognized that plant-symbiotic fungi are supported by photosynthates, but little is known about the mechanisms for fungi to utilize plant-derived carbon sources. ABSTRACT It is widely recognized that plant-symbiotic fungi are supported by photosynthates; however, little is known about the molecular mechanisms underlying the utilization of plant-derived sugars by rhizospheric fungi. In the insect-pathogenic and plant-symbiotic fungus Metarhizium robertsii, we previously showed that the utilization of oligosaccharides by the transporter MRT (Metarhizium raffinose transporter) is important for rhizosphere competency. In this study, we identified a novel monosaccharide transporter (MST1) that is involved in the colonization of the rhizoplane and acts additively with MRT to colonize the rhizosphere. MST1 is not involved in infection of insects by M. robertsii. MST1 is an H+ symporter and is able to transport a broad spectrum of monosaccharides, including glucose, sorbose, mannose, rhamnose, and fructose. Deletion of the Mst1 gene impaired germination and mycelial growth in medium containing the sugars that it can transport. Homologs of MST1 were widely found in many fungi, including plant symbionts such as Trichoderma spp. and mycorrhizal fungi and plant pathogens such as Fusarium spp. This work significantly advances insights into the development of symbiotic relationships between plants and fungi. IMPORTANCE Over 90% of all vascular plant species develop an intimate symbiosis with fungi, which has an enormous impact on terrestrial ecosystems. It is widely recognized that plant-symbiotic fungi are supported by photosynthates, but little is known about the mechanisms for fungi to utilize plant-derived carbon sources. In the fungus Metarhizium robertsii, we identified a novel monosaccharide transporter (MST1) that is an H+ symporter and can transport a broad spectrum of monosaccharides, including glucose, sorbose, mannose, rhamnose, and fructose. MST1 is involved in the colonization of the rhizoplane and acts additively with the previously characterized oligosaccharide transporter MRT to colonize the rhizosphere. Homologs of MST1 were found in many fungi, including plant symbionts and plant pathogens, suggesting that the utilization of plant-derived sugars by MST1 homologs could also be important for other fungi to develop a symbiotic or parasitic relationship with their respective plant hosts.