SsCox17, a copper chaperone, is required for pathogenic process and oxidative stress tolerance of Sclerotinia sclerotiorum
SsCox17, a copper chaperone, is required for pathogenic process and oxidative stress tolerance of Sclerotinia sclerotiorum
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DOI：
10.1016/j.plantsci.2022.111345
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发表时间：
2022-06-09
期刊：
影响因子：
5.2
通讯作者：
Qian, Wei
中科院分区：
文献类型：
--
作者：
Ding, Yijuan;Chen, Yangui;Qian, Wei
由核盘菌（Sclerotinia sclerotiorum）引起的茎腐病是世界油菜的主要病原真菌之一。致病性的发展是微妙地依赖于活性氧（ROS）的调节。Cox17是将铜离子从细胞质运送到线粒体进行细胞色素c氧化酶（CCO）组装的关键因子。目前，没有关于Cox17在真菌发病机制中的影响的数据。本研究旨在从功能上研究Cox17在S.菌核病发病机理SsCox17转录本在油菜上的接种过程中表现出较高的表达水平。在SsCox17基因沉默的菌株中，线粒体内铜含量和CCO活性降低。SsCox17基因表达上调的菌丝在氧化胁迫和缺陷反应检测到SsCox17基因沉默菌株的氧化胁迫。与S.与野生型菌株相比，SsCox17基因沉默菌株的毒力随之降低。进一步发现SsCox17过表达菌株增加了铜含量、CCO活性、对氧化胁迫的耐受性和毒力。我们还观察到附着胞的形成和SsCox17有一定的相关性。这些结果为SsCox17与真菌毒力和氧化解毒正相关提供了证据。
Stem rot, caused by Sclerotinia sclerotiorum has emerged as one of the major fungal pathogens of oilseed Brassica across the world. The pathogenic development is exquisitely dependent on reactive oxygen species (ROS) modulation. Cox17 is a crucial factor that shuttles copper ions from the cytosol to the mitochondria for the cytochrome c oxidase (CCO) assembly. Currently, no data is available regarding the impact of Cox17 in fungal pathogenesis. The present research was carried out to functionally characterize the role of Cox17 in S. sclerotiorum pathogenesis. SsCox17 transcripts showed high expression levels during inoculation on rapeseed. Intramitochondrial copper content and CCO activity were decreased in SsCox17 gene-silenced strains. The SsCox17 gene expression was up-regulated in the hyphae under oxidative stress and a deficiency response to oxidative stress was detected in SsCox17 gene-silenced strains. Compared to the S. sclerotiorum wild-type strain, there was a concomitant reduction in the virulence of SsCox17 gene-silenced strains. The SsCox17 overexpression strain was further found to increase copper content, CCO activity, tolerance to oxidative stress and virulence. We also observed a certain correlation of appressoria formation and SsCox17. These results provide evidence that SsCox17 is positively associated with fungal virulence and oxidative detoxification.