miRNA editing landscape reveals miR-34c regulated spermatogenesis through structure and target change in pig and mouse
miRNA editing landscape reveals miR-34c regulated spermatogenesis through structure and target change in pig and mouse
复制标题
miRNA 编辑景观揭示 miR-34c 通过结构和靶标变化调节猪和小鼠的精子发生
DOI：
10.1016/j.bbrc.2018.05.197
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发表时间：
2018
影响因子：
3.1
通讯作者：
Liao Mingzhi
中科院分区：
文献类型：
--
作者：
Wang Xiaodan;Zhang Peng;Li Leijie;Che Dongxue;Li Tongtong;Li Hao;Li Qun;Jia Haiyang;Tao Shiheng;Hua Jinlian;Zeng Wenxian;Liao Mingzhi
精子发生与男性不育密切相关。MicroRNAs(MiRNAs)在精子发生过程中对靶基因的调控起着至关重要的作用。世界各地高通量测序的海量数据集为挖掘神秘的分子机制提供了基础。但如何利用大数据，挖掘miRNA的调控仍然是一个具有挑战性的问题。在这里，我们整合了精子发生的转录组，发现miRNA通过编辑miRNA来调节精子发生。然后对不同物种进行了比较，发现在精子发生过程中，miRNA编辑位点数目和编辑类型在不同细胞类型之间的分布是保守的。有趣的是，我们进一步发现，近一半的编辑事件发生在小鼠和猪的种子区域。最后，我们发现miR-34c在精子发生的各个阶段都被频繁地编辑，它通过RNA结构的变化来调节其靶基因，并在编辑时表现出功能障碍。综上所述，我们描述了小鼠和猪精子发生过程中miRNA编辑的总体概况，并揭示了miR-34c可能通过miRNA编辑发挥作用。
Spermatogenesis has a close relationship with male infertility. MicroRNAs (miRNAs) play crucial roles in their regulation of target genes during spermatogenesis. A huge dataset of high-throughput sequencing all over the world provides the basis to dig the cryptic molecular mechanism. But how to take advantage of the big data and unearth the miRNA regulation is still a challenging problem. Here we integrated transcriptome of spermatogenesis and found miRNA regulate spermatogenesis through miRNA editing. We then compared different species and found that the distributions of miRNA editing site number and editing types among different cell types during spermatogenesis are conservative. Interesting, we further found that nearly half of the editing events occurred in the seed region in both mouse and pig. Finally, we foundmiR-34c, which is edited frequently at all stages during spermatogenesis, regulates its target genes through the RNA structure changing and shows dysfunction when it is edited. Summary, we depicted the overall profile of miRNA editing during spermatogenesis in mouse and pig and reveal miR-34c may play its roles through miRNA editing.