EFFECTS OF PHYLLOSILICATE CLAY ON THE METABOLIC PROFILE OF AFLATOXIN B-1 IN FISCHER-344 RATS
EFFECTS OF PHYLLOSILICATE CLAY ON THE METABOLIC PROFILE OF AFLATOXIN B-1 IN FISCHER-344 RATS
复制标题
DOI：
10.1016/0378-4274(94)03179-b
复制
发表时间：
1995-01-01
影响因子：
3.5
通讯作者：
PHILLIPS, TD
中科院分区：
文献类型：
--
作者：
SARR, AB;MAYURA, K;PHILLIPS, TD
页硅酸盐粘土、水合铝硅酸钠钙 (HSCAS) 已被证明可以通过降低黄曲霉毒素的生物利用度来预防农场动物的黄曲霉中毒。本研究旨在确定 HSCAS 对黄曲霉毒素敏感物种中黄曲霉毒素 B-1 (AFB(1)) 代谢的影响。雄性Fischer-344大鼠单独口服1.0、0.5、0.25和0.125 mg AFB(1)/kg体重以及与0.5% HSCAS组合。 6、24、36和48小时后收集尿液样本。无论有或没有 HSCAS，大多数尿液样本中均检测到黄曲霉毒素 M(1) (AFM(1)) 和黄曲霉毒素 P-1 (AFP(1))。发现 AFM(1) 是主要代谢物。 HSCAS 存在时，代谢物浓度显着降低，更重要的是，没有检测到额外的代谢物。我们的结果表明 AFB(1)-HSCAS 复合物在体内没有显着解离，并支持 HSCAS 与黄曲霉毒素紧密结合的早期发现，
The phyllosilicate clay, hydrated sodium calcium aluminosilicate (HSCAS), has been shown to prevent aflatoxicosis in farm animals by reducing the bioavailability of aflatoxin. The present study was designed to determine the effects of HSCAS on the metabolism of aflatoxin B-1 (AFB(1)) in an aflatoxin-sensitive species. Male Fischer-344 rats were orally dosed with 1.0, 0.5, 0.25 and 0.125 mg AFB(1)/kg body weight alone and in combination with 0.5% HSCAS. Urine samples were collected after 6, 24, 36, and 48 h. Aflatoxin M(1) (AFM(1)) and aflatoxin P-1 (AFP(1)) were detected in most urine samples, with or without HSCAS. AFM(1) was found to be the major metabolite. Metabolite concentrations were significantly decreased in the presence of HSCAS, and more importantly, no additional metabolites were detected. Our results suggest that the AFB(1)-HSCAS complex was not significantly dissociated in vivo, and support earlier findings that HSCAS tightly binds aflatoxin,