Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis
Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis
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PSK1（一种来自牡丹皮的 SKP1 样基因同源物）的过度表达赋予拟南芥耐盐性。
DOI：
10.1007/s00299-016-2066-z
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发表时间：
2017-01-01
影响因子：
6.2
通讯作者：
Shu, Qingyan
中科院分区：
文献类型：
--
作者：
Hao, Qing;Ren, Hongxu;Shu, Qingyan
我们的研究是第一个证明PSK 1，SKP 1样基因同源物，参与耐盐性。本研究从牡丹中克隆了一个S期激酶相关蛋白1（SKP 1）的同源基因，命名为PSK 1。PSK 1开放阅读框为462 bp，编码153个氨基酸，分子量为17 kDa。该基因全长1,634 bp，包含一个904 bp的内含子。PSK 1基因在所有组织中均有表达，其中在萼片中表达量最高，其次是叶片。在盐胁迫下，PSK 1在拟南芥中的过表达导致相对于野生型植物的发芽率、子叶绿化和鲜重增加。此外，含有35 S：：PSK 1的转基因拟南芥系显示出盐胁迫下生殖和生长所必需的基因表达增加：ASK 1，LEAFY，FT和CO参与花发育和开花时间，以及P5 CS，RAB 18，DREB和SOD 1 -3有助于耐盐性。我们对PSK 1的功能表征增加了对先前探索的SKP 1样基因在植物中的多种功能的全面了解，并揭示了其在耐盐性中作用的分子机制。本研究为进一步阐明PSK 1在牡丹花发育中的功能及其分子机制提供了信息，为牡丹开花调控和耐盐性的获得提供了理论依据。
Our study is the first to demonstrate that PSK1 , a SKP1 -like gene homologue, is involved in salinity tolerance. Our functional characterization of PSK1 provides new insights into tree peony development.A homologous gene of S-phase kinase-associated protein1 (SKP1) was cloned from tree peony (Paeonia suffruticosa) and denoted as PSK1. The 462-bp open reading frame of PSK1 was predicted to encode a protein comprising 153 amino acids, with a molecular mass of 17 kDa. The full-length gene was 1,634 bp long and included a large 904-bp intron. PSK1 transcription was detected in all tissues, with the highest level observed in sepals, followed by leaves. Under salinity stress, overexpression of PSK1 in Arabidopsis resulted in increased germination percentages, cotyledon greening, and fresh weights relative to wild-type plants. Furthermore, transgenic Arabidopsis lines containing 35S::PSK1 displayed increased expression of genes that would be essential for reproduction and growth under salinity stress: ASK1, LEAFY, FT, and CO involved in flower development and flowering time as well as P5CS, RAB18, DREB, and SOD1-3 contributing to salinity tolerance. Our functional characterization of PSK1 adds to global knowledge of the multiple functions of previously explored SKP1-like genes in plants and sheds light on the molecular mechanism underlying its role in salinity tolerance. Our findings also provide information on the function and molecular mechanism of PSK1 in tree peony flower development, thereby revealing a theoretical basis for regulation of flowering and conferral of salinity tolerance in tree peony.