EFFECTS OF F-MET-LEU-PHE-INDUCED INFLAMMATION ON INTESTINAL LYMPH-FLOW AND LYMPHATIC PUMP BEHAVIOR
EFFECTS OF F-MET-LEU-PHE-INDUCED INFLAMMATION ON INTESTINAL LYMPH-FLOW AND LYMPHATIC PUMP BEHAVIOR
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DOI：
10.1152/ajpgi.1992.262.2.g199
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发表时间：
1992-02-01
影响因子：
--
通讯作者：
ZAWIEJA, DC
中科院分区：
文献类型：
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作者：
BENOIT, JN;ZAWIEJA, DC
文献中的先前研究表明，肠内放置趋化肽N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸（f-Met-Leu-Phe）引起以间质液积聚和淋巴流量增加为特征的肠道炎症反应。 此外，已知淋巴液远离肠的运动依赖于通过在肠系膜中收集淋巴液而有节奏地泵送淋巴液。 本研究的目的是确定f-Met-Leu-Phe诱导的淋巴形成增加是否被淋巴泵效率的增加所抵消。 将雄性-Sprague-道利大鼠麻醉，并将具有相邻肠系膜的回肠段取出。 肠系膜位于光学窗口上方，选择100 μ m收集淋巴管进行研究。 将制备物转移至视频显微镜，并在对照条件下和在1-μ-M f-Met-Leu-Phe的管腔内输注期间监测淋巴泵的活性。 通过每搏输出量和收缩频率的乘积计算淋巴泵的淋巴推进。 在一组动物中，通过对回肠段的淋巴引流进行插管来确定总淋巴流量。 在肠中放置f-Met-Leu-Phe后，总淋巴流量增加。 淋巴流量的增加被淋巴泵的增加所抵消。 淋巴泵引起的淋巴推进的增加完全是由于每搏输出量增加，因为收缩频率没有改变。 本研究的结果表明，激活淋巴泵在急性炎症可能是重要的，在防止间质水肿。
Previous studies in the literature indicate that intraenteric placement of the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe) evokes an intestinal inflammatory response characterized by an accumulation of interstitial fluid and increased lymph flow. Furthermore, it is known that movement of lymph away from the intestine is dependent on the rhythmic pumping of lymph by collecting lymphatics in the mesentery. The purpose of the present study was to determine whether the f-Met-Leu-Phe-induced increase in lymph formation is countered by an increase in lymphatic pump efficiency. Male-Sprague-Dawley rats were anesthetized, and a segment of ileum with adjacent mesentery was exteriorized. The mesentery was positioned over an optical window, and a 100-mu-m collecting lymphatic was selected for study. The preparation was transferred to a video microscope, and the activity of the lymphatic pump was monitored under control conditions and during intraluminal infusion of 1-mu-M f-Met-Leu-Phe. Lymph propulsion by the lymphatic pump was calculated from the product of stroke volume and contraction frequency. In one group of animals, total lymph flow was determined by cannulating the lymphatic draining the ileal segment. Total lymph flow increased following f-Met-Leu-Phe placement in the intestine. The increased lymph flow was paralleled by a rise in lymphatic pumping. The rise in lymph propulsion by the lymphatic pump resulted exclusively from an increased stroke volume, inasmuch as contraction frequency did not change. The results of the present study suggest that activation of the lymphatic pump during acute inflammation may be important in preventing interstitial edema.