Aberrant axonal projections from mammillary bodies in Pax6 mutant mice : possible roles of Netrin-1 and Slit2 in mammillary projections
Aberrant axonal projections from mammillary bodies in Pax6 mutant mice : possible roles of Netrin-1 and Slit2 in mammillary projections
复制标题
Pax6突变小鼠乳头体的异常轴突投射：Netrin-1和Slit2在乳头投射中的可能作用
DOI：
10.1002/jnr.21966
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发表时间：
2009
影响因子：
4.2
通讯作者：
Takahashi H
中科院分区：
文献类型：
--
作者：
Tsuchiya R;Takahashi K;Liu F-C;Takahashi H
哺乳动物小体形成轴索的早期事件仍鲜为人知。在目前的研究中，我们报道了缺乏转录因子Pax6的小鼠从乳头体到丘脑背侧的轴突投射的异常模式。我们发现，在Pax6突变小鼠的背侧丘脑中，Netrin-1基因异位上调，Slit1和Slit2基因下调。然后，我们通过宫内电穿孔技术研究了Netrin-1和Slit2对哺乳动物轴突的影响。Netrin-1对哺乳动物轴突具有诱人的作用。此外，在Netrin-1基因缺陷的小鼠中，哺乳动物的轨迹是混乱的。另一方面，Slit2对哺乳动物轴突有排斥作用。这些发现表明，Netrin和Sit的结合可能参与了哺乳动物小体的正确轴突投射，它们在间脑中的错误表达可能导致了Pax6突变小鼠这些轴突的错误路线。©2008 Wiley-Liss，Inc.
Early events in the axonal tract formation from mammillary bodies remain poorly understood. In the present study, we reported an aberrant pattern of axonal projections from mammillary bodies to the dorsal thalamus in mice lacking the transcription factor Pax6. We found thatNetrin‐1was ectopically up‐regulated and that bothSlit1andSlit2were down‐regulated in the presumptive dorsal thalamus of Pax6 mutant mice. We then examined the effects of Netrin‐1 and Slit2 on the mammillary axons by in utero electroporation techniques. Netrin‐1 had an attractive action toward the mammillary axons. Moreover, mammillary trajectories were disorganized in Netrin‐1‐deficient mice. On the other hand, Slit2 had a repulsive effect on the mammillary axons. These findings suggest that the combination of Netrin and Slit may be involved in proper axonal projection from the mammillary bodies and that their misexpression in the diencephalon may cause the misrouting of these axons in Pax6 mutant mice. © 2008 Wiley‐Liss, Inc.