Precise Tuning and Characterization of Viscoelastic Interfaces for the Study of Early Epithelial-Mesenchymal Transition Behaviors
Precise Tuning and Characterization of Viscoelastic Interfaces for the Study of Early Epithelial-Mesenchymal Transition Behaviors
复制标题
DOI：
10.1021/acs.langmuir.1c03048
复制
发表时间：
2022-02-10
期刊：
影响因子：
3.9
通讯作者：
Nakanishi, Jun
中科院分区：
文献类型：
--
作者：
Chang, Alice Chinghsuan;Uto, Koichiro;Nakanishi, Jun
越来越多的证据表明细胞功能受到周围基质的粘弹性的调节。本研究旨在研究界面粘弹性对上皮细胞粘附和上皮间质转化（EMT）行为的影响。使用由ε-己内酯和D,L-丙交酯与二苯甲酮光交联的共聚物组成的旋涂薄膜来控制界面粘弹性，并使用原子力显微镜和流变仪表征其机械性能。上皮 Madin-Darby 犬肾 (MDCK) 细胞形态转变的临界范围约为 102ms 弛豫时间，比报道的弛豫时间 (10-102s) 小 1-2 个数量级。对应变率相关的粘弹性特性的分析表明，差异是由用于界面和本体机械表征的不同应变率/频率引起的。此外，界面粘性项和弹性项的解耦表明，E/N-钙粘蛋白表达水平受到界面松弛和弹性的不同调节。这些结果证实了界面粘弹性的精确操控和表征在 EMT 进展的力学生物学研究中的重要性
There is growing evidence that cellular functions are regulated by the viscoelastic nature of surrounding matrices. This study aimed toinvestigate the impact of interfacial viscoelasticity on adhesion and epithelial-mesenchymal transition (EMT) behaviors of epithelial cells. The interfacialviscoelasticity was manipulated using spin-coated thinfilms composed ofcopolymers of epsilon-caprolactone andD,L-lactide photo-cross-linked withbenzophenone, whose mechanical properties were characterized using atomicforce microscopy and a rheometer. The critical range for the morphologicaltransition of epithelial Madin-Darby canine kidney (MDCK) cells was of theorder of 102ms relaxation time, which was 1-2 orders of magnitude smallerthan the relaxation times reported (10-102s). An analysis of strain rate-dependent viscoelastic properties revealed that the difference was caused bythe different strain rate/frequency used for the mechanical characterization ofthe interface and bulk. Furthermore, decoupling of the interfacial viscous and elastic terms demonstrated that E/N-cadherinexpression levels were regulated differently by interfacial relaxation and elasticity. These results confirm the significance of precisemanipulation and characterization of interfacial viscoelasticity in mechanobiology studies on EMT progression