Bioenergetic properties of alkalophilic Bacillus sp. strain C-59 on an alkaline medium containing K2CO3
Bioenergetic properties of alkalophilic Bacillus sp. strain C-59 on an alkaline medium containing K2CO3
复制标题
嗜碱芽孢杆菌的生物能特性。
DOI：
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发表时间：
1987
影响因子：
3.2
通讯作者：
K. Horikoshi
中科院分区：
文献类型：
--
作者：
M. Kitada;K. Horikoshi
嗜碱芽孢杆菌C-59在含K_2CO_3和Na_2CO_3的碱性培养基上生长良好，但在贫K ~+培养基上不生长。右侧出膜囊泡，通电的情况下，Na+，但是，不能采取[14 C]甲胺积极，而囊泡与10 mM NaCl平衡积极采取[14 C]甲胺。[14 C]丝氨酸的摄取也被刺激的Na+的加入，和施加的钠梯度引起瞬时摄取。这些结果表明，Na+/H+逆向转运蛋白参与pH稳态和产生的电化学钠梯度的菌株C-59，即使对Na+的生长要求是不明显的。22 Na+从22 Na +-加载囊泡流出更快，在pH 9.5比在pH 7的电子供体的存在下。另一方面，囊泡在pH 7显示出更快的流出比在pH 9.5时，反向转运蛋白通电的缬氨霉素介导的K+扩散电位（内负）。
Alkalophilic Bacillus sp. strain C-59 could grow well on an alkaline medium containing K2CO3, as well as Na2CO3, but did not grow on K+-depleted medium. Right-side-out membrane vesicles, energized in the absence of Na+, however, could not take up [14C]methylamine actively, while vesicles equilibrated with 10 mM NaCl actively took up [14C]methylamine. The uptake of [14C]serine was also stimulated by the addition of Na+, and the imposition of a sodium gradient caused transient uptake. These results indicated that an Na+/H+ antiporter was involved in pH homeostasis and generation of an electrochemical sodium gradient in strain C-59 even though a growth requirement for Na+ was not evident. The efflux of 22Na+ from 22Na+-loaded vesicles was more rapid at pH 9.5 than at pH 7 in the presence of an electron donor. On the other hand, vesicles at pH 7 showed more rapid efflux than at pH 9.5 when the antiporter was energized by a valinomycin-mediated K+ diffusion potential (inside negative).
体内嗜碱杆菌 Na /H 逆向转运蛋白的表征：全细胞中 δ psi 依赖性 22Na 流出。
影响因子：
3.2
作者：
Garcia,ML;Guffanti,AA;Krulwich,TA
通讯作者：
Krulwich,TA
呼吸产生的跨膜电位不等于坚强芽孢杆菌RAB合成ATP时相同大小的扩散电位。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Guffanti,AA;Fuchs,RT;Schneier,M;Chiu,E;Krulwich,TA
通讯作者：
Krulwich,TA