Microarray analysis reveals early responsive genes possibly involved in localized nitrate stimulation of lateral root development in maize (Zea mays L.)
Microarray analysis reveals early responsive genes possibly involved in localized nitrate stimulation of lateral root development in maize (Zea mays L.)
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DOI：
10.1016/j.plantsci.2008.04.009
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发表时间：
2008-09-01
期刊：
影响因子：
5.2
通讯作者：
Mi, Guohua
中科院分区：
文献类型：
--
作者：
Liu, Jinxin;Han, Lili;Mi, Guohua
耕地土壤养分空间分布极不均匀。侧根在硝酸盐丰富的土壤中增殖是有据可查的。然而，关于哪些基因参与这一过程的信息却很少，特别是在谷物中。为了了解玉米（Zea mays L.）局部硝酸盐诱导LR生长的分子机制，我们利用玉米寡核苷酸阵列（Maize Oligonucleotide Array）（http：//www.example.com）和分根系统分析了玉米根对局部硝酸盐刺激的早期反应（1 h）中的基因表达谱。www.maizearray.org通过半定量RT-PCR进一步证实了选定的差异表达基因。结果表明，局部NO3-信号的接收和/或传递涉及一些重要的蛋白激酶和蛋白磷酸酶（组氨酸激酶）。丝氨酸/苏氨酸激酶、蛋白磷酸酶2A等）和转录因子（F-box、锌指、Myb和bZIP转录因子和应答调节因子）。生长素反应因子7 b，乙烯受体，细胞分裂素氧化酶编码基因的表达增加表明激素途径和当地NO3-信号通路之间的强相互作用。涉及NO3-吸收和同化的基因（NRT 2.1，NR等），糖运输（糖转运蛋白）和利用（蔗糖合成酶）在氮营养根增强。此外，局部NO3-诱导与细胞分裂和扩增相关的基因的快速表达，例如a-扩张蛋白、纤维素合成酶、驱动蛋白、质膜和液泡膜水通道蛋白。基于差异表达的基因，提出了一个结合“NO3信号”和“代谢库”理论的推定模型来解释控制玉米局部硝酸盐诱导LR伸长的分子机制。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
Arable soils are extremely heterogeneous in spatial nutrient distribution. It is well documented that lateral roots (LRs) proliferate in nitrate-rich patch. Yet less information is available as to which genes are involved in this process, in particular in cereals. To understand the molecular mechanism for local nitrate-induced LR growth in maize (Zea mays L.), we analyzed the gene expression profiling in maize root in early response (1 h) to local nitrate stimulation by using Maize Oligonucleotide Array (http:// www.maizearray.org) and a split-root system. Selected differentially expressed genes were further confirmed by semi-quantitative RT-PCR. The results showed that reception and/or transduction of local NO3- signal involve some important protein kinases and protein phosphatases (histidine kinases. serine/ threonine kinases, protein phosphatase 2A, etc.) and transcription factors (F-box, Zinc finger, Myb and bZIP transcription factors and response regulator). Increasing expression of genes encoding auxin response factor 7b, ethylene receptor, and cytokinin oxidase suggests strong interaction among hormonal pathways and local NO3- signaling pathways. Genes involving NO3- uptake and assimilation (NRT2.1, NR, etc.), sugar transport (a sugar transporter) and utilization (a sucrose synthase) were enhanced in the N-fed root. Furthermore, local NO3- induces rapid expression of genes related to cell division and expansion such as a-expansin, cellulose synthase, kinesin, plasma membrane and tonoplast aquaporins. Based on the differentially expressed genes, a putative model which combines both the "NO3- signal" and "metabolic sink" theories is proposed to explain the molecular mechanism controlling local nitrate-induced LR elongation in maize. (c) 2008 Elsevier Ireland Ltd. All rights reserved.