Bat-transmitted human rabies outbreaks, Brazilian Amazon
Bat-transmitted human rabies outbreaks, Brazilian Amazon
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DOI：
10.3201/eid1708.050929
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发表时间：
2006-08-01
影响因子：
11.8
通讯作者：
Vasconcelos, Pedro F. C.
中科院分区：
文献类型：
--
作者：
da Rosa, Elizabeth S. T.;Kotait, Ivanete;Vasconcelos, Pedro F. C.
我们描述了巴西北部帕拉州波特尔市和维塞乌市偏远农村地区发生的两起蝙蝠传播的疫情。患者死亡后采集中枢神经系统标本，进行狂犬病抗原免疫荧光检测和组织病理学检查；此外，还将样本注射到乳鼠脑内，试图分离病毒。对获得的菌株进行了抗原和遗传特征分析。这两个城市有 21 人死于麻痹性狂犬病。从人体标本中分离出 10 种狂犬病病毒株；另外2例经组织病理学检查确诊。分离株经抗原鉴定为 Desmodus rotundus 变体 3 (AgV3)。对 6 个菌株的 DNA 测序表明，它们在基因上与巴西分离的 D. rotundus 相关菌株接近。遗传结果与使用单克隆抗体获得的结果相似，并支持这样的结论：所研究的分离株属于同一狂犬病周期，即在吸血蝙蝠 D. rotundus 中发现的病毒变种。
We describe 2 bat-transmitted outbreaks in remote, rural areas of Portel and Viseu Municipalities, Para State, northern Brazil. Central nervous system specimens were taken after patients' deaths and underwent immunofluorescent assay and histopathologic examination for rabies antigens; also, specimens were injected intracerebrally into suckling mice in an attempt to isolate the virus. Strains obtained were antigenically and genetically characterized. Twenty-one persons died due to paralytic rabies in the 2 municipalities. Ten rabies virus strains were isolated from human specimens; 2 other cases were diagnosed by histopathologic examination. Isolates were antigenically characterized as Desmodus rotundus variant 3 (AgV3). DNA sequencing of 6 strains showed that they were genetically close to D. rotundus-related strains isolated in Brazil. The genetic results were similar to those obtained by using monoclonal antibodies and support the conclusion that the isolates studied belong to the same rabies cycle, the virus variants found in the vampire bat D. rotundus.