Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective Criteria
Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective Criteria
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DOI：
10.1007/s12155-012-9222-2
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发表时间：
2013-03-01
影响因子：
3.6
通讯作者：
Nascimento, Maurcio Andrade
中科院分区：
文献类型：
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作者：
Nascimento, Iracema Andrade;Izabel Marques, Sheyla Santa;Nascimento, Maurcio Andrade
藻类生物柴油工业的可行性取决于选择适当的菌株，考虑到有利可图的产量和油的质量。本工作旨在生物勘探和筛选12个微藻菌株的应用，作为选择标准，体积脂质生产力和脂肪酸谱，用于估计生物柴油燃料的性质。不同菌株的体积脂质生产率从22.61到204.91 mg l（-1）天（-1）不等。观察到小球藻（204.91 mg l（-1）天（1））和葡萄球菌菌株（布朗葡萄球菌和可怕葡萄球菌分别为112.43和98.00 mg l（-1）天（-1））的脂质产量最高。聚类和主成分分析应用于脂肪酸甲酯（FAME）配置文件区分三个不同的微藻群体，根据其生产生物柴油的潜力。Kirchneriella lunaris、Ankistrodesmus fusiformis、Chlamydocapsa bacillus和Ankistrodesmus falcatus表现出最高水平的多不饱和FAME，其在生物柴油的生产中引起最低的十六烷值（CN）（42.47-50.52）、最高的碘值（IV）（101.33-136.97）和最低的氧化稳定性。衣原体和栅藻油中饱和FAME含量较高，表明它们是具有较高氧化稳定性、较高CN（63.63-64.94）和较低IV（27.34-35.28）的生物柴油来源。第三组，除了出现在隔离Trebouxyophyceae菌株，是由微藻产生的CN，IV和氧化稳定性的中间值的生物柴油，其饱和和单饱和脂质的水平。因此，在这项研究中，FAME分析表明，产生高质量微藻生物柴油的最佳方法是混合不同细胞培养物的油。
The viability of algae-based biodiesel industry depends on the selection of adequate strains in regard to profitable yields and oil quality. This work aimed to bioprospecting and screening 12 microalgae strains by applying, as selective criteria, the volumetric lipid productivity and the fatty acid profiles, used for estimating the biodiesel fuel properties. Volumetric lipid productivity varied among strains from 22.61 to 204.91 mg l(-1) day(-1). The highest lipid yields were observed for Chlorella (204.91 mg l(-1) day(1)) and Botryococcus strains (112.43 and 98.00 mg l(-1) day(-1) for Botryococcus braunii and Botryococcus terribilis, respectively). Cluster and principal components analysis analysis applied to fatty acid methyl esters (FAME) profiles discriminated three different microalgae groups according to their potential for biodiesel production. Kirchneriella lunaris, Ankistrodesmus fusiformis, Chlamydocapsa bacillus, and Ankistrodesmus falcatus showed the highest levels of polyunsaturated FAME, which incurs in the production of biodiesels with the lowest (42.47-50.52) cetane number (CN), the highest (101.33-136.97) iodine values (IV), and the lowest oxidation stability. The higher levels of saturated FAME in the oils of Chlamydomonas sp. and Scenedesmus obliquus indicated them as source of biodiesel with higher oxidation stability, higher CN (63.63-64.94), and lower IV (27.34-35.28). The third group, except for the Trebouxyophyceae strains that appeared in isolation, are composed by microalgae that generate biodiesel of intermediate values for CN, IV, and oxidation stability, related to their levels of saturated and monosaturated lipids. Thus, in this research, FAME profiling suggested that the best approach for generating a microalgae-biodiesel of top quality is by mixing the oils of distinct cell cultures.