In vivo footprinting of specific protein-DNA interactions.
In vivo footprinting of specific protein-DNA interactions.
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特定蛋白质-DNA 相互作用的体内足迹。
DOI：
10.1016/0076-6879(87)52077-8
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发表时间：
1987
影响因子：
--
通讯作者：
G. Felsenfeld
中科院分区：
文献类型：
--
作者：
P. Jackson;G. Felsenfeld
在这一章中，作者描述了一种在近单碱基分辨率下绘制复杂基因组中特定独特DNA序列内切割模式的方法。该方法已被用来准确地定位鸡细胞和细胞核内..帽α...和..β.-珠蛋白基因家族中调控序列上的序列特异性蛋白质-核酸相互作用的位置。他们检查了通过外源添加的核酸酶引入到..β..和..capal..-珠蛋白基因启动子的基因组拷贝中的切割模式，但任何切割DNA的试剂都是这种分析的候选试剂。该方法可以应用于任何需要体内结构信息的基因的研究。这里描述的方法是先前发表的方法的改进版本。
The authors describe in this chapter a method for mapping the pattern of cleavages within specific unique DNA sequences in a complex genome at near single base resolution. The method has been used to accurately map the sites of sequence-specific protein-nuclei acid interactions upon regulatory sequences in various members of the ..cap alpha..- and ..beta..-globin gene families within chicken cells and cell nuclei. They examined the pattern of cleavages introduced into the genomic copies of ..beta..- and ..cap alpha..-globin gene promoters by exogenously added nuclease, but any reagent that cleaves DNA is a candidate for this analysis. The method can be applied to the study of any gene for which in vivo structural information is desirable. The method described here is an improved version of that previously published.
纯化的金霉素发色团导致脱氧核糖核酸链断裂时需要还原剂。
影响因子：
2.9
作者：
Kappen,LS;Napier,MA;Goldberg,IH;Samy,TS
通讯作者：
Samy,TS