Biosynthesis of polyhydroxybutyrate in Enterobacter sp. SEL2 and Enterobacteriaceae bacterium sp. PFW1 using sugar cane molasses as media
Biosynthesis of polyhydroxybutyrate in Enterobacter sp. SEL2 and Enterobacteriaceae bacterium sp. PFW1 using sugar cane molasses as media
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肠杆菌属中聚羟基丁酸酯的生物合成。
DOI：
10.5897/ajb11.1405
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
G. Abbas
中科院分区：
文献类型：
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作者：
N. Naheed;N. Jamil;S. Hasnain;G. Abbas
以甘蔗糖蜜为原料，研究了其作为生产聚羟基丁酸酯（PHB）的培养基。采用不同稀释度的甘蔗糖蜜与水和无机盐培养基组合，并在不同的碳源和氮源比例下使用。在从三种类型的有机废物污染环境中分离的54种细菌中，通过尼罗蓝A活菌落染色法选择两种在UV下具有最高荧光强度的细菌，并且它们在摇瓶培养中在pH 7.0、37°C和150 rpm下从葡萄糖产生最大量的PHB（66.61±0.05%和76.92±0.04%）。细菌菌株被鉴定为革兰氏阴性杆菌，16 S核糖体核糖核酸（RNA）序列相似性搜索显示与肠杆菌属的同源性为99%和98%。和肠杆菌科细菌。在肠杆菌属（Enterobacter sp.）SEL 2（JF 901810）和肠杆菌科细菌PFW 1（JF 901811）在pH 7.0、37°C和150 rpm条件下，聚羟基丁酸酯（PHB）的生物合成率分别为57.61±0.57%和58.07±0.25%。菌株SEL 2和PFW 1的最适培养时间分别为24 ~ 48 h和48 ~ 72 h。在矿物培养基中含有2%糖蜜和0.2%硫酸铵的培养基（培养基11）中观察到最佳生长和聚羟基链烷酸酯（PHA）生产。提取的PHA颗粒的功能基团被确定为C=O基团通过傅立叶变换红外（FTIR）光谱分析和质子核磁共振（NMR）分析证实它是一个羟基丁酸酯的均聚物。
关键词：聚羟基脂肪酸酯（PHA），苏丹黑B，尼罗蓝A，糖蜜，聚羟基丁酸酯（PH B），肠杆菌，肠杆菌科。
The present research was conducted to check the cane molasses as a media for the production of polyhydroxybutyrate (PHB). Different dilutions of cane molasses were used in combination with water and mineral medium and in different ratios of carbon and nitrogen sources. Out of the 54 bacteria isolated from three types of organic waste contaminated environments, two were selected for their highest intensity of fluorescence under UV by Nile blue A viable colony staining method and they produced maximum amount of PHB from glucose (66.61±0.05% and 76.92±0.04%) in a shake flask culture at pH 7.0, 37°C and 150 rpm. The bacterial strains were identified as Gram negative rods and the 16S ribosomal ribonucleic acid (RNA) sequence similarity search showed 99 and 98% homology to Enterobacter sp. andEnterobacteriaceae bacterium respectively. In bacterial strains Enterobacter sp. SEL2 (JF901810) and Enterobacteriaceae bacterium PFW1 (JF901811), the polyhydroxybutyrate (PHB) biosynthesis from molasses was observed as 57.61±0.57% and 58.07±0.25% respectively at pH 7.0, 37°C and 150 rpm. The optimal time was 24 to 48 h for strain SEL2 and 48 to 72 h for strain PFW1. The best growth and polyhydroxyalkanoates (PHA) production was observed in media with 2% molasses and 0.2% ammonium sulphate in mineral medium (Media 11). The functional groups of the extracted PHA granules were identified as C=O group by Fourier transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance (NMR) analysis confirmed it as a homopolymer of hydroxybutyrate.
Key words: Polyhydroxyalkanoates (PHA), Sudan Black B, Nile blue A, molasses, polyhydroxybutyrate (PHB), Enterobacter, Enterobacteriaceae.