Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin
Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin
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DOI：
10.1007/s00436-005-1368-9
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发表时间：
2005-08-01
影响因子：
2
通讯作者：
Jiménez-Cardoso, E
中科院分区：
文献类型：
--
作者：
Eligio-García, L;Cortes-Campos, A;Jiménez-Cardoso, E
在几种动物身上发现人贾第虫表明存在人畜共患传染。我们研究了G。肠道分离株来自：腹泻儿童（n=6）、无症状儿童（n=7）、无菌培养物（n=7）和犬（n=11）。PCR扩增16 S rRNA序列，测序，并与基因库中A、B基因型和Dog基因型进行比较。结果表明，9/20（45%）的儿童分离株属于基因型A和11/20（55%）显示一些可变位点，允许在三个任意聚类：A1，A2和A3分类。另外，7/11（63%）的狗分离株是A型，包括那些与儿童生活在同一地区的狗，而4/11（37%）属于生活在不同地区的任意A4簇。在这项研究中，基因型A与儿童和狗的样本相关，因此，我们可以推断人畜共患病传播是获得疾病的一种方式。
The presence of human Giardia in several animals suggests a zoonotic transmission. We studied G. Intestinalis isolates obtained from: children with diarrhea (n=6), asymptomatic children (n=7), axenic cultures (n=7) and dogs (n=11). The sequence corresponding to 16 S rRNA was amplified by PCR, sequenced and compared with genotypes A, B and Dog sequences reported in the Gene Bank database. Results show that 9/20 (45%) of children isolates belonged to genotype A and 11/20 (55%) showed some variable sites, allowing classification in three arbitrary clusters: A1, A2 and A3. In adition 7/11 (63%) of dog isolates were genotype A, including those dogs that lived in the same locality as the children lived, while 4/11 (37%) belonged to an arbitrary A4 cluster living in a different locality. In this study, genotype A was associated with samples from children and dogs, and, therefore, we could infer zoonotic transmission as a way of getting the disease.