Photochemical reactions of microcrystalline thymidine.
Photochemical reactions of microcrystalline thymidine.
复制标题
微晶胸苷的光化学反应。
DOI：
10.1021/ol5036276
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发表时间：
2015
期刊：
影响因子：
5.2
通讯作者：
Li,Lei
中科院分区：
文献类型：
--
作者：
Jian,Yajun;Ames,DavidM;Ouyang,Hao;Li,Lei
由于在给定时间存在多种构象异构体，核苷/核苷酸/寡核苷酸光反应通常会同时产生多种产物。如此复杂的反应模式使得人们很难专注于单个 DNA 光产物并阐明其形成的要求。据报道，微晶中胸苷光反应的一个罕见例子，其中 5-胸腺嘧啶基-5,6-二氢胸腺嘧啶，例如孢子光产物（SP），作为主要物种产生，产率约 85%。这种前所未有的高产量为未来 SP 光化学详细研究扫清了主要障碍。
Nucleoside/nucleotide/oligonucleotide photoreactions usually result in a number of products simultaneously due to a wide range of conformers existing at a given time. Such a complicated reaction pattern makes it difficult for one to focus on a single DNA photoproduct and elucidate the requirements for its formation. A rare example of thymidine photoreaction in microcrystals is reported, where 5-thyminyl-5,6-dihydrothymine, e.g., the spore photoproduct (SP), is produced as the dominant species in ∼85% yield. This unprecedented high yield clears the major obstacle for future SP photochemistry studies in detail.