Inactivation of Trypanosoma cruzi trypomastigote forms in blood components with a Psoralen and Ultraviolet A light.
Inactivation of Trypanosoma cruzi trypomastigote forms in blood components with a Psoralen and Ultraviolet A light.
复制标题
用补骨脂素和紫外线 A 光灭活血液成分中的克氏锥虫锥鞭毛体。
DOI：
10.1111/j.1751-1097.1996.tb05656.x
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发表时间：
1996
影响因子：
3.3
通讯作者：
Ben-Hur,E
中科院分区：
文献类型：
--
作者：
Gottlieb,P;Margolis-Nunno,H;Robinson,R;Shen,LG;Chimezie,E;Horowitz,B;Ben-Hur,E
研究了UVA和4′-氨甲基-4，5 ′，8-三甲基丙戊酸钠（AMT）对新鲜冰冻血浆（FFP）和浓缩血小板（PC）血液成分中克氏锥虫的灭活作用。AMT的使用浓度为50 μg/mL，灭活程序包括类黄酮芦丁（0.35 mM），这是一种I型和II型光反应物的猝灭剂，我们之前发现其在该治疗方案期间可维持血小板完整性。在FFP和PC中，完全灭活了T的感染形式。cruzi，锥虫鞭毛体，在4.2 J/cm 2的UVA（320-400 nm辐射）能量密度下获得。我们注意到，虽然寄生虫的感染性在4.2 J/cmZ下被消除，但锥虫鞭毛体的运动性在处理后至少持续16 h，并且仅在高得多的光剂量后才被抑制。在存在3 H-AMT的情况下处理后，从寄生虫细胞中分离总DNA表明，在致死UVA通量下，每个双酶对产生约0.5个AMT加合物。这些结果表明，这种peptide加UVA的方法，这表明在提高PC的病毒安全性的承诺，可以另外消除bloodleT。克氏锥虫病的病原体。
Inactivation of the blood‐borne parasiteTrypanosoma cruziby UVA and 4′‐aminomethyl‐4,5′,8‐trimethylpsor‐alen (AMT) was studied in the blood components fresh frozen plasma (FFP) and platelet concentrate (PC). The AMT was utilized at a concentration of 50 μg/mL and the inactivation procedure included the flavonoid rutin (at 0.35 mM), a quencher of type I and type II photo‐reactants, which we have previously found to maintain platelet integrity during this treatment regimen. Within both FFP and PC, complete inactivation of the infective form ofT. cruzi, the trypomastigote, was achieved at a UVA (320–400 nm radiation) fluence of 4.2 J/cm2. We note that while the infectivity of the parasite is eliminated at 4.2 J/cmZthe trypomastigote motility continues for at least 16 h post‐treatment and is inhibited only after much higher light doses. Isolation of total DNA from the parasite cells after treatment in the presence of3H‐AMT indicated that at the lethal UVA fluence about 0.5 AMT adducts per kilobase pairs occurred. These results suggest that this psoralen plus UVA methodology, which shows promise in enhancing the viral safety of PC, may in addition eliminate bloodborneT. cruzi, the causative agent of Chagas disease.