Unequivocal imaging of aluminium in human cells and tissues by an improved method using morin.
Unequivocal imaging of aluminium in human cells and tissues by an improved method using morin.
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通过使用桑色素的改进方法对人体细胞和组织中的铝进行明确成像。
DOI：
10.1007/s00418-019-01809-0
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发表时间：
2019
影响因子：
2.3
通讯作者：
Mold MJ
中科院分区：
文献类型：
--
作者：
Mold MJ
铝具有生物反应性，其增强免疫反应的能力促使其被纳入兽医和人类疫苗中。因此，需要明确的可视化铝在体内创造了一个集中的研究工作，以建立荧光分子探针为此目的。最常用的用于检测铝的直接荧光标记物是桑色素（2′，3，4 ′，5，7-五羟基黄酮）和Lumogallion [4-氯-3-（2，4-二羟基苯偶氮）-2-羟基苯-1-磺酸]。虽然前者在检测植物中的铝（主要是根尖中的铝）方面得到了普及，但后者在检测人体细胞和组织中的铝方面具有更高的灵敏度和选择性。在此，我们开发了一种简化的桑色素染色协议，使用自发荧光猝灭剂，苏丹黑B。这种修改后的方案改善了组织形态并提高了分析灵敏度，这使得在单核细胞中以及在与诊断为家族性阿尔茨海默病的供体的人脑组织中的老年斑共定位时能够检测到细胞内铝。总的来说，我们的研究结果表明，一个简单的方法，以尽量减少假阳性使用桑色素明确检测铝在体内。
Aluminium is biologically reactive and its ability to potentiate the immune response has driven its inclusion in both veterinary and human vaccines. Consequently, the need for unequivocal visualisation of aluminium in vivo has created a focused research effort to establish fluorescent molecular probes for this purpose. The most commonly used direct fluorescent labels for the detection of aluminium are morin (2′,3,4′,5,7-pentahydroxyflavone) and lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid]. While the former has gained popularity in the detection of aluminium in plants and predominantly within root tips, the latter boasts greater sensitivity and selectivity for the detection of aluminium in human cells and tissues. Herein, we have developed a simplified morin staining protocol using the autofluorescence quenching agent, Sudan Black B. This modified protocol improves tissue morphology and increases analytical sensitivity, which allows intracellular aluminium to be detected in monocytes and when co-localised with senile plaques in human brain tissue of donors diagnosed with familial Alzheimer’s disease. Overall, our results demonstrate a simple approach to minimise false positives in the use of morin to unequivocally detect aluminium in vivo.