3-ARM NUCLEIC-ACID JUNCTIONS ARE FLEXIBLE
3-ARM NUCLEIC-ACID JUNCTIONS ARE FLEXIBLE
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DOI：
10.1093/nar/14.24.9745
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发表时间：
1986-12-22
影响因子：
14.9
通讯作者：
SEEMAN, NC
中科院分区：
文献类型：
--
作者：
MA, RI;KALLENBACH, NR;SEEMAN, NC
核酸连接是生命系统中短暂存在的分支 DNA 结构的稳定类似物。我们在这里表明，包含三个双螺旋臂的连接可以使用传统的粘端连接程序进行酶促寡聚化，以产生更大的复合物。该产品由一系列由 20 个碱基对分隔的连接点组成。形成的连接二聚体仅具有游离末端，而三聚体和更大的种类被发现是未闭合的和环化的。令人惊讶的是，一系列大环产物的形成以三聚体和四聚体开始，表明该连接点绕弯曲轴是柔性的，并且可能也是扭转的。我们从三个不同的三臂连接处获得了相同的结果，其中两个连接处的侧面有 3 个 Watson-Crick 碱基对，另一种的连接处的侧面有一个 G-G 碱基对。
Nucleic acid junctions are stable analogs of branched DNA structures which occur transiently in living systems. We show here that junctions which contain three double helical arms can be enzymatically oligomerized, using conventional sticky-ended ligation procedures, to create larger complexes. The products consist of a series of linked junctions separated by 20 base pairs. Junction dimers are formed that have free termini only, whereas trimers and larger species are found to be both unclosed and cyclized. The formation of a series of macrocyclic products which, surprisingly, begins with trimers and tetramers indicates that this junction is flexible about a bending axis, and perhaps twist-wise as well. We have obtained the same results from three different 3-arm junctions, two in which the junction is flanked by a 3 Watson-Crick base pairs, and one in which a G-G base pair flanks the junction.