GROWTH OF ANTARCTIC CYANOBACTERIA UNDER ULTRAVIOLET-RADIATION - UVA COUNTERACTS UVB INHIBITION
GROWTH OF ANTARCTIC CYANOBACTERIA UNDER ULTRAVIOLET-RADIATION - UVA COUNTERACTS UVB INHIBITION
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DOI：
10.1111/j.0022-3646.1995.00242.x
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发表时间：
1995-04-01
影响因子：
2.9
通讯作者：
VINCENT, WF
中科院分区：
文献类型：
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作者：
QUESADA, A;MOUGET, JL;VINCENT, WF
从南极冰架池塘中分离到一株垫状蓝藻(Phormidium Murrayi West And West)，在白光下结合一定范围的WA光和WE光进行培养。随着紫外线(UV)辐射的增加，4天的生长率下降，对WE的响应比UVA大9倍。体内吸收光谱显示，UVA和UVB在更大程度上引起藻蓝蛋白/叶绿素a的降低和类胡萝卜素/叶绿素a的升高，藻蓝蛋白/叶绿素a的比值与UVB抑制的生长速率密切正相关。在固定的紫外辐射光谱梯度下，生长抑制作用以UVB为主。然而，在特定的UVB辐射下，生长的抑制依赖于UVB/UVA的比值，并且生长速率随着Wa的增加而线性增加。这些结果与我们的观点是一致的，即我们的抑制代表了损伤和修复过程之间的平衡，这两个过程都由不同的波段控制。他们还强调了在实验室和现场紫外线毒性检测中考虑紫外线光谱平衡的必要性。
A mat-forming cyanobacterium (Phormidium murrayi West and West) isolated from an ice-shelf pond in Antarctica was grown under white light combined with a range of WA and WE irradiances. The 4-day growth rate decreased under increasing ultraviolet (UV) radiation, with a ninefold greater response to WE relative to UVA. In vivo absorbance spectra showed that UVA and to a greater extent UVB caused a decrease in phycocyanin/chlorophyll a and an increase in carotenoids/chlorophyll a. The phycocyanin/chlorophyll a ratio was closely and positively correlated to the UVB-inhibited growth rate. Under fixed spectral gradients of UV radiation, the growth inhibition effect was dominated by UVB. However, at specific UVB irradiances the inhibition of growth depended on the ratio of UVB to UVA, and growth rates increased linearly with increasing WA. These results are consistent with the view that WE inhibition represents the balance between damage and repair processes that are each controlled by separate wavebands. They also underscore the need to consider UV spectral balance in laboratory and field assays of UVB toxicity.