Inactivation of Kell blood group antigens by 2‐aminoethylisothiouronium bromide
Inactivation of Kell blood group antigens by 2‐aminoethylisothiouronium bromide
复制标题
2-氨基乙基异硫脲溴化物灭活凯尔血型抗原
DOI：
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发表时间：
1982
影响因子：
6.5
通讯作者：
W. Marsh
中科院分区：
文献类型：
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作者：
H. Advani;J. Zamor;W. Judd;C. Johnson;W. Marsh
用含有6% 2-氨乙基-异硫脲溴铵（AET）的溶液孵育的人红细胞失去了Kell血型系统的一部分或相关抗原的活性。然而，Kx抗原未失活。对广泛的其他血型抗原的研究显示，没有其他变化的证据，AET似乎与具有Kell活性的红细胞膜结构发生特异性反应。AET程序产生可用于血型血清学的人工K 0红细胞，并允许容易地识别与Kell系统相关的抗体。
Human red cells incubated with a solution containing 6% 2‐aminoethyl‐isothiouronium bromide (AET) lose activity of antigens that are part of, or related to, the Kell blood group system. However, Kx antigen is not inactivated. Studies on a wide range of other blood‐group antigens show no other evidence of changes and AET appears to react specifically with red‐cell membrane structures that have Kell activity. The AET procedure produces an artificial K0 red cell that can be used in blood group serology, and allows easy recognition of antibodies that are associated with the Kell system.