Organogenesis from dissociated cells: Generation of mature cycling hair follicles from skin-derived cells
Organogenesis from dissociated cells: Generation of mature cycling hair follicles from skin-derived cells
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DOI：
10.1111/j.0022-202x.2005.23716.x
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发表时间：
2005-05-01
影响因子：
6.5
通讯作者：
Stenn, KS
中科院分区：
文献类型：
--
作者：
Zheng, Y;Du, XB;Stenn, KS
毛囊的形成和循环涉及上皮成分和间质成分之间广泛且持续的相互作用。这里描述了一种从分离的上皮细胞和间充质细胞快速、可重复地生成毛囊的系统。该系统既可作为测量细胞的毛原特性的工具，又可作为研究分离细胞组装器官机制的工具。在这个系统中，当从新生小鼠皮肤中分离出的分离细胞被注射到成年小鼠躯干皮肤中时，毛囊就会发育。这种形态发生过程涉及上皮细胞聚集形成簇，这些簇通过细胞凋亡塑造形成“漏斗状囊肿”。从“漏斗状囊肿”中，毛胚离心形成，随后形成毛芽，然后不对称生长以分化成循环成熟的毛囊皮脂腺结构。标记研究将这些卵泡的分子分化与原位系统相关联。这项研究表明，上皮-间质系统发育的最早阶段——即使是从分离的细胞制剂中——也需要上皮平台。
Hair follicle formation and cycling involve extensive and continuous interactions between epithelial and mesenchymal components. A system for rapidly and reproducibly generating hair follicles from dissociated epithelial and mesenchymal cells is described here. The system serves both as a tool for measuring the trichogenic property of cells and as a tool for studying the mechanisms that dissociated cells use to assemble an organ. In this system, hair follicles develop when dissociated cells, isolated from newborn mouse skin, are injected into adult mouse truncal skin. This morphogenetic process involves the aggregation of epithelial cells to form clusters that are sculpted by apoptosis to generate "infundibular cysts". From the "infundibular cysts", hair germs form centrifugally followed by follicular buds and then pegs that grow asymmetrically to differentiate into cycling mature pilosebaceous structures. Marker studies correlate the molecular differentiation of these follicles with in situ systems. This study suggests that the earliest phase of a developing epithelial-mesenchymal system-even from dissociated cell preparations-requires an epithelial platform.