Enhanced osteogenic differentiation of human bone-derived mesenchymal stem cells in 3-dimensional printed porous titanium scaffolds by static magnetic field through up-regulating Smad4
Enhanced osteogenic differentiation of human bone-derived mesenchymal stem cells in 3-dimensional printed porous titanium scaffolds by static magnetic field through up-regulating Smad4
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DOI：
10.1096/fj.201802195r
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发表时间：
2019-05-01
期刊：
影响因子：
4.8
通讯作者：
Qiu, Guixing
中科院分区：
文献类型：
--
作者：
He, Yu;Yu, Lingjia;Qiu, Guixing
对于骨科医生来说，大型骨缺损的重建仍然是一个巨大的挑战。三维打印支架被认为是一种很有前途的修复材料。静磁场治疗是一种有效的、非侵入性的促进骨再生的治疗方法。然而，SMF在3DP支架中对人骨髓间充质干细胞(HBMSCs)的成骨作用及其可能的机制尚不清楚。本研究在体外和体内研究了SMF对人骨髓间充质干细胞在3DP支架上的成骨作用。此外，通过蛋白质组学分析，探讨了其促进成骨的可能机制。结果表明，SMF在体外可促进hBMSCs向成骨细胞分化。通过蛋白质组学分析，在SMF条件下共鉴定出185个差异蛋白质。其成骨作用可能与骨形态发生蛋白Smad1/5/8信号通路和SMF条件下上调Smad4使磷酸化Smad1/5/8和磷酸化Smad2/3转运增加有关。体内实验表明，SMF对大鼠骨缺损模型有促进骨再生和骨结合的作用。结论：适量SMF是一种安全有效的体外和体内促进3DP支架成骨的方法。He，Y.，Yu，L.，Liu，J.，Li，Y.，Wu，Y.，Huang，Z.，Wu，D.，Wang，H.，Wu，Z.，Chu，G.在静磁场作用下，通过上调Smad4，促进人骨源性间充质干细胞在三维打印多孔钛支架中的成骨分化。
The reconstruction of large bone defects remains a significant challenge for orthopedists. Three-dimensional-printed (3DP) scaffold is considered a promising repair material. Static magnetic field (SMF) treatment is an effective and noninvasive therapeutic method to improve bone regeneration. However, the osteogenic effect of SMF on human bone-derived mesenchymal stem cells (hBMSCs) in 3DP scaffolds, as well as its potential mechanism, are unclear. In this study, the osteogenic effect of SMF on hBMSCs in a 3DP scaffold was investigated in vitro and in vivo. In addition, the potential mechanism for promoting osteogenesis was investigated by proteomic analysis. The results showed that SMF promoted osteogenic differentiation of hBMSCs in vitro. A total of 185 differential proteins were identified under SMF conditions by proteomic analysis. The osteogenic effect might be associated with bone morphogenetic protein-Smad1/5/8-signaling pathway and increased transport of phosphorylated Smad1/5/8 and phosphorylated Smad2/3 to the nucleus by up-regulating Smad4 under SMF conditions. The in vivo experiment showed that bone regeneration and osseointegration was enhanced by SMF in the rat model of bone defect. In conclusion, moderate SMF was a safe and effective method for enhancing osteogenesis in 3DP scaffolds in vitro and in vivo.He, Y., Yu, L., Liu, J., Li, Y., Wu, Y., Huang, Z., Wu, D., Wang, H., Wu, Z., Qiu, G. Enhanced osteogenic differentiation of human bone-derived mesenchymal stem cells in 3-dimensional printed porous titanium scaffolds by static magnetic field through up-regulating Smad4.