FimY does not interfere with FimZ-FimW interaction during type 1 fimbria production by Salmonella enterica serovar Typhimurium.
FimY does not interfere with FimZ-FimW interaction during type 1 fimbria production by Salmonella enterica serovar Typhimurium.
复制标题
在肠沙门氏菌鼠伤寒沙门氏菌产生 1 型菌毛过程中，FimY 不会干扰 FimZ-FimW 相互作用。
DOI：
10.1128/iai.00795-13
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发表时间：
2013
影响因子：
3.1
通讯作者：
Clegg,Steven
中科院分区：
文献类型：
--
作者：
Zeiner,SarahA;Dwyer,BrettE;Clegg,Steven
鼠伤寒沙门氏菌中 1 型菌毛的产生部分由三种蛋白 FimZ、FimY 和 FimW 控制。氨基酸序列分析表明FimZ属于双组分系统细菌反应调节剂家族。在这些研究中，我们证明引入模拟 FimZ 磷酸化的突变对于激活其靶基因 fimA 是必要的。此外，仅当 FimZ 被磷酸化时，FimZ 与 FimW（imA 表达的阻遏物）才会发生相互作用。因此，FimW 的负调节作用很可能是由于活性 FimZ 蛋白的下调所致。 FimY 似乎不具有响应调节剂的功能，并且它的活性可以通过模仿 FimY 的磷酸化而丧失。 FimY 的过量产生不能减轻 FimZ 突变体中的非流苏表型，而高水平的 FimZ 可以克服 FimY 突变体中的非流苏表型。看来FimY 在FimZ 的上游起作用以激活fimA 表达。
The production of type 1 fimbriae in Salmonella enterica serovar Typhimurium is controlled, in part, by three proteins, FimZ, FimY, and FimW. Amino acid sequence analysis indicates that FimZ belongs to the family of bacterial response regulators of two-component systems. In these studies, we have demonstrated that introducing a mutation mimicking phosphorylation of FimZ is necessary for activation of its target gene,fimA. In addition, the interaction of FimZ with FimW, a repressor offimAexpression, occurs only when FimZ is phosphorylated. Consequently, the negative regulatory effect of FimW is most likely due to downmodulation of the active FimZ protein. FimY does not appear to function as a response regulator, and its activity can be lost by mimicking the phosphorylation of FimY. Overproduction of FimY cannot alleviate the nonfimbriate phenotype in a FimZ mutant, whereas high levels of FimZ can overcome the nonfimbriate phenotype of a FimY mutant. It appears that FimY acts upstream of FimZ to activatefimAexpression.