Production of recombinant thanatin in watery rice seeds that lack an accumulation of storage starch and proteins
Production of recombinant thanatin in watery rice seeds that lack an accumulation of storage starch and proteins
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DOI：
10.1016/j.jbiotec.2015.12.006
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发表时间：
2016-02-10
影响因子：
4.1
通讯作者：
Shimada, Hiroaki
中科院分区：
文献类型：
--
作者：
Imamura, Tomohiro;Sekine, Ken-Taro;Shimada, Hiroaki
分子农业是生产具有商业价值的材料的一种有前途的方法。植物有望成为重组蛋白生产的合适宿主。然而，转基因植物生产在实际应用之前必须解决两大挑战：产物积累不足和纯化方法难以建立。我们提出了一种使用缺乏储存淀粉和蛋白质积累的水状水稻种子生产所需蛋白质的简单程序，这是通过引入反义 SPK 诱导的表型。我们培育出一种转基因水稻植物，其中含有抗菌肽 Thanatin 以及反义 SPK 的基因。生物测定和蛋白质组分析表明，重组thanatin在这些水性水稻种子中以活性形式积累。这些结果表明我们的系统对于塔那亭的生产有效。与叶子生产相比，该过程可以轻松去除杂质并简化纯化过程。因此，我们的系统可能是生产所需材料（包括蛋白质）的有用技术。 (C) 2015 Elsevier B.V. 保留所有权利。
Molecular farming is a promising method for producing materials of commercial interest. Plants can be expected to be appropriate hosts for recombinant protein production. However, production in genetically modified plants has two major challenges that must be resolved before its practical use: insufficient accumulation of products and difficulty in establishing methods for their purification. We propose a simple procedure for the production of a desired protein using watery rice seeds lacking an accumulation of storage starch and proteins, a phenotype induced by the introduction of an antisense SPK. We produced a transgenic rice plant containing a gene for an antimicrobial peptide, thanatin, together with antisense SPK. Bioassay and proteome analysis indicated that recombinant thanatin accumulated in an active form in these watery rice seeds. These results suggest that our system worked effectively for the production of thanatin. This procedure enabled easy removal of impurities and simplified the purification process compared with production in leaves. Our system may therefore be a useful technique for the production of desired materials, including proteins. (C) 2015 Elsevier B.V. All rights reserved.