Determination of the degree of polymerisation of fructans from ryegrass and chicory using MALDI-TOF Mass Spectrometry and Gel Permeation Chromatography coupled to multiangle laser light scattering
Determination of the degree of polymerisation of fructans from ryegrass and chicory using MALDI-TOF Mass Spectrometry and Gel Permeation Chromatography coupled to multiangle laser light scattering
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DOI：
10.1016/j.foodhyd.2015.01.015
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发表时间：
2016-02
影响因子：
10.7
通讯作者：
M. Evans;J. Gallagher;I. Ratcliffe;Peter A. Williams
中科院分区：
文献类型：
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作者：
M. Evans;J. Gallagher;I. Ratcliffe;Peter A. Williams
测定了菊苣和黑麦草(Lolium perenneL.)果聚糖的聚合度。使用凝胶渗透色谱与多角度激光光散射和折射率检测器(GPC/MALLS)和基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF MS)联用。结果表明，从黑麦草中分离出的果聚糖的聚合度在2-100之间，而从菊苣中提取的商品化生产的果聚糖的聚合度在2-61之间。研究表明，MALDI-TOF MS在测定低摩尔质量物质的熔点时特别有效，但在检测高熔点分子的存在时效果较差。另一方面，GPC/MALLS能够提供更大范围的DP值，尽管它在非常低的DP值时不那么敏感。结果表明，这两种技术提供了互补的信息，从而提供了更准确的果聚糖分子总等电点的估计。
This study is concerned with the determination of the degree of polymerisation (DP) of fructans from chicory and rye-grass (Lolium perenneL.) using Gel Permeation Chromatography coupled to multiangle laser light scattering and refractive index detectors (GPC/MALLS) and Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry (MALDI-TOF MS). The results show that fructans isolated from ryegrass have a DP in the range 2–>100 and the commercially produced fructans derived from chicory have a DP in range 2–61. It has been demonstrated that MALDI-TOF MS is particularly effective at determining the DP of low molar mass material but is less effective for detecting the presence of high DP molecules. On the other hand GPC/MALLS is able to provide a much broader range of DP values although it is less sensitive at very low DP. It has been shown that the two techniques give complementary information thus providing a more accurate estimate of the overall DP of the fructan molecules.