Transcription factor VqERF114 regulates stilbene synthesis in Chinese wild Vitis quinquangularis by interacting with VqMYB35
Transcription factor VqERF114 regulates stilbene synthesis in Chinese wild Vitis quinquangularis by interacting with VqMYB35
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转录因子VqERF114通过与VqMYB35相互作用调节中国野生五角葡萄中二苯乙烯的合成
DOI：
10.1007/s00299-019-02456-4
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发表时间：
2019-10-01
影响因子：
6.2
通讯作者：
Wang, Yuejin
中科院分区：
文献类型：
--
作者：
Wang, Lan;Wang, Yuejin
VqERF114通过与VqMYB35相互作用调节芪的合成。白藜芦醇是一种二苯乙烯，是一类重要的次生代谢产物，在一些植物物种中积累，包括葡萄藤。在植物中，它们参与对植物病原体攻击的反应，作为人类饮食的一部分，它们提供了一系列重大的健康益处。芪合酶（STS），负责白藜芦醇合成的关键酶，已在少数植物物种的特点。然而，芪合成的调节机制是不确定的。在这里，ERF家族转录因子从中国野生葡萄属毛葡萄，VqERF 114，其特征在于作为一个间接调节芪的合成。葡萄藤叶片中VqERF114的瞬时过表达测定导致STS表达和芪积累增加。然而，VqERF114不结合VqSTS的启动子，但MYB转录因子VqMYB35确实与VqERF114相互作用。这种相互作用证实了酵母双杂交试验和双分子荧光互补。此外，VqMYB 35通过直接与VqSTS 15、VqSTS 28、VqSTS 42和VqSTS 46启动子中的MBS元件结合，对它们的表达具有激活作用。VqERF114和VqMYB35的共过表达导致更高的VqSTS表达和更多的二苯乙烯合成。这些结果表明VqERF114通过与VqMYB35相互作用来调节芪合成。
VqERF114 regulates stilbene synthesis by interacting with VqMYB35. Resveratrol is a stilbene, an important class of secondary metabolites that accumulates in some plant species, including grapevine. In the plant, these are involved in the response to attack by plant pathogens and, as a component of the human diet, they offer a range of significant health benefits. Stilbene synthase (STS), the key enzyme responsible for resveratrol synthesis, has been characterised in a small number of plant species. However, the regulatory mechanisms for stilbene synthesis are uncertain. Here, an ERF family transcription factor from Chinese wild Vitis quinquangularis, VqERF114, was characterised as an indirect regulator of stilbene synthesis. A transient overexpression assay of VqERF114 in grapevine leaves led to increased STS expression and stilbene accumulation. However, VqERF114 did not bind to the promoters of VqSTSs but the MYB transcription factor, VqMYB35, did interact with VqERF114. This interaction was confirmed by a yeast two-hybrid assay and bimolecular fluorescence complementation. Furthermore, VqMYB35 showed activation effects on the expressions of VqSTS15, VqSTS28, VqSTS42 and VqSTS46 by binding directly to the MBS elements in their promoters. Co-overexpression of VqERF114 and VqMYB35 resulted in higher VqSTSs expression and more stilbene synthesis. These results demonstrate that VqERF114 regulates stilbene synthesis by interacting with VqMYB35.