Transcutaneous immunization using a dissolving microneedle array protects against tetanus, diphtheria, malaria, and influenza
Transcutaneous immunization using a dissolving microneedle array protects against tetanus, diphtheria, malaria, and influenza
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DOI：
10.1016/j.jconrel.2012.04.001
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发表时间：
2012-06-28
影响因子：
10.8
通讯作者：
Nakagawa, Shinsaku
中科院分区：
文献类型：
--
作者：
Matsuo, Kazuhiko;Hirobe, Sachiko;Nakagawa, Shinsaku
与常用的注射系统相比，经皮免疫（TCI）是一种有吸引力的替代接种途径。我们以前开发了一种溶解微针阵列用作TCI装置，并报告了具有溶解微针阵列的TCI诱导针对模型抗原的免疫应答。在本研究中，我们调查了使用这种疫苗接种系统预防破伤风和白喉、疟疾和流感的疫苗接种效果。我们的TCI系统诱导类毒素特异性IgG水平和毒素中和抗体滴度的大幅增加，并诱导抗SE36 IgG的产生，其可以结合疟疾寄生虫。在流感HA疫苗接种时，在小鼠中引发了稳健的抗体产生，其提供了针对随后的流感病毒攻击的完全保护。这些发现表明，使用溶解微针阵列的TCI可以引发针对感染性疾病的大的免疫应答。基于这些结果，我们现在正在为人类临床试验准备转化研究。(C)2012爱思唯尔有限公司版权所有。
Transcutaneous immunization (TCI) is an attractive alternative vaccination route compared to the commonly used injection systems. We previously developed a dissolving microneedle array for use as a TCI device, and reported that TCI with the dissolving microneedle array induced an immune response against model antigens. In the present study, we investigated the vaccination efficacy against tetanus and diphtheria, malaria, and influenza using this vaccination system. Our TCI system induced substantial increases in toxoid-specific IgG levels and toxin-neutralizing antibody titer and induced the production of anti-SE36 IgG, which could bind to malaria parasite. On influenza HA vaccination, robust antibody production was elicited in mice that provided complete protection against a subsequent influenza virus challenge. These findings demonstrate that TCI using a dissolving microneedle array can elicit large immune responses against infectious diseases. Based on these results, we are now preparing translational research for human clinical trials. (C) 2012 Elsevier B.V. All rights reserved.