Multiscale Mechanical Simulations of Cell Compacted Collagen Gels
Multiscale Mechanical Simulations of Cell Compacted Collagen Gels
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DOI：
10.1115/1.4024460
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发表时间：
2013-07-01
影响因子：
1.7
通讯作者：
Sander, E. A.
中科院分区：
文献类型：
--
作者：
Aghvami, Maziar;Barocas, V. H.;Sander, E. A.
工程组织通常被拉伸或压缩（即，在培养过程中调节）以刺激细胞外基质（ECM）产生并改善生长构建体的机械性质。然而，机械刺激和ECM重塑之间的关系是复杂的、相互依赖的和动态的。因此，需要理论模型来理解潜在的现象，以便可以优化调节过程以产生功能性工程组织。在这里，我们继续我们的多尺度力学模型的发展，通过模拟细胞牵引的影响，发展等距张力和重新分配力的周围纤维的胶原凝胶嵌入外植体。该模型预测的纤维重组模式与实验观察到的模式相似。此外，与无细胞情况相比，包含细胞压实也改变了凝胶中纤维应变的分布，特别是在发现最高应变的细胞周围区域。
Engineered tissues are commonly stretched or compressed (i.e., conditioned) during culture to stimulate extracellular matrix (ECM) production and to improve the mechanical properties of the growing construct. The relationships between mechanical stimulation and ECM remodeling, however, are complex, interdependent, and dynamic. Thus, theoretical models are required for understanding the underlying phenomena so that the conditioning process can be optimized to produce functional engineered tissues. Here, we continue our development of multiscale mechanical models by simulating the effect of cell tractions on developing isometric tension and redistributing forces in the surrounding fibers of a collagen gel embedded with explants. The model predicted patterns of fiber reorganization that were similar to those observed experimentally. Furthermore, the inclusion of cell compaction also changed the distribution of fiber strains in the gel compared to the acellular case, particularly in the regions around the cells where the highest strains were found.