Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p
Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p
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DOI：
10.1101/gad.10.24.3081
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发表时间：
1996-12-15
影响因子：
10.5
通讯作者：
Koshland, D
中科院分区：
文献类型：
--
作者：
CohenFix, O;Peters, JM;Koshland, D
后期的启动被认为是通过一种未知的抑制物的泛素依赖的蛋白分解来控制的。这一过程涉及后期促进复合体(APC)，这是一种特定的泛素连接酶，已被证明参与有丝分裂周期蛋白的降解。以往的研究表明，在酿酒酵母中，Pds1蛋白是一种后期抑制因子，提示它可能是APC的靶标。在这里，我们表明在酵母细胞和有丝分裂非洲爪哇提取物中，Pds1p以APC依赖的方式被降解。此外，Pds1p直接被非洲爪哇APC泛化。在芽生酵母中，Pds1p在后期开始时被降解，Pds1p的不可降解衍生物抑制后期的开始。我们得出结论，Pds1p是一种后期抑制物，其依赖于APC的降解是后期启动所必需的。
Anaphase initiation has been postulated to be controlled through the ubiquitin-dependent proteolysis of an unknown inhibitor. This process involves the anaphase promoting complex (APC), a specific ubiquitin ligase that has been shown to be involved in mitotic cyclin degradation. Previous studies demonstrated that in Saccharomyces cerevisiae, Pds1 protein is an anaphase inhibitor and suggested that it may be an APC target. Here we show that in yeast cells and in mitotic Xenopus extracts Pds1p is degraded in an APC-dependent manner. In addition, Pds1p is directly ubiquitinated by the Xenopus APC. In budding yeast Pds1p is degraded at the time of anaphase initiation and nondegradable derivatives of Pds1p inhibit the onset of anaphase. We conclude that Pds1p is an anaphase inhibitor whose APC-dependent degradation is required for the initiation of anaphase.